Anti-inflammatory effect of non-thermal atmospheric pressure plasma for periodontitis treatment: in vitro pilot study

치주염 치료를 위한 저온상압 플라즈마의 항염효과: 예비 실험

  • Park, You li (Department of Periodontology, School of Dentistry and Dental Research Institute, Pusan National University) ;
  • Kim, Hyun-Joo (Department of Periodontology, School of Dentistry and Dental Research Institute, Pusan National University) ;
  • Lee, Ju-Youn (Department of Periodontology, School of Dentistry and Dental Research Institute, Pusan National University) ;
  • Jeong, Sung-Hee (Department of Oral Medicine, School of Dentistry and Dental Research Institute, Pusan National University) ;
  • Kwon, Eun-Young (Dental Clinic Center, Pusan National University Hospital) ;
  • Joo, Ji-Young (Department of Periodontology, School of Dentistry and Dental Research Institute, Pusan National University)
  • 박유리 (부산대학교 치의학전문대학원 치주과학교실) ;
  • 김현주 (부산대학교 치의학전문대학원 치주과학교실) ;
  • 이주연 (부산대학교 치의학전문대학원 치주과학교실) ;
  • 정성희 (부산대학교 치의학전문대학원 구강내과학교실) ;
  • 권은영 (부산대학교병원 치과진료센터) ;
  • 주지영 (부산대학교 치의학전문대학원 치주과학교실)
  • Received : 2021.03.11
  • Accepted : 2021.05.21
  • Published : 2021.06.30


Purpose: The purpose of this study was to evaluate the anti-inflammatory effects of non-thermal atmospheric pressure plasma (NTP) on human gingival fibroblasts (HGFs) for clinical application of periodontal treatment. Materials and Methods: HGFs were treated with Porphyromonas gingivalis (Pg) lipopolysaccharide (LPS). Customized NTP device was developed for periodontal in vitro study. Cell viability was evaluated with cell counting kit-8. The levels of inflammatory cytokines, including interleukin (IL)-8 and 6, were determined by enzyme-linked immunosorbent assay. Results: When NTP was applied, the cell viability did not change significantly, and there was no difference for 6 h and 24h. When Pg LPS was treated to HGFs, the secretion of IL-8 and IL-6 was increased compared to the control group. But when the NTP was applied, the secretion of them was significantly decreased. Conclusion: NTP did not affect cell viability of HGFs. And it inhibited the LPS-induced production of IL-8 and IL-6.

목적: 대표적인 만성 구강내 염증성 질환인 치주염의 치료에 저온상압 플라즈마의 적용 가능성을 평가해 보고자, 치주 조직내 많이 분포하고 있는 치은섬유모세포에 대한 항염효과를 평가해보았다. 연구 재료 및 방법: 두명의 환자의 구강내에서 건강한 치은조직을 채득하여 primary culture 시행한 후 실험실에서 치은섬유모세포를 계대배양하였다. 세포 실험을 위해 헬륨 가스를 이용하는 저온상압 플라즈마 장치를 제작하였다. 치주염증을 유도하기 위해 Porphyromonas gingivalis (Pg)의 LPS를 처리하고, CCK-8 kit를 통해 세포생존율 평가를 시행하고 염증성 사이토카인인 interleukin (IL)-8, IL-6의 분비정도를 평가하였다. 결과: 인간치은섬유모세포 생존율 평가에서는 Pg LPS를 처리한 군과 플라즈마를 처리한 군, Pg LPS와 플라즈마를 함께 처리한 군 모두에서 생존율이 92.28%에서 100% 사이로 존재하고 통계적으로 유의성은 관찰되지 않았다. 6시간과 24시간 세포배양시간에 따른 세포생존율 차이도 관찰되지 않았다. Pg LPS를 적용하였을 때 대조군에 비해 IL-8과 6의 분비가 증가되었으며, 저온상압 플라즈마의 적용시 그 분비가 통계적으로 유의하게 감소되었다. 결론: 저온상압 플라즈마가 인간섬유모세포의 세포 생존율에 유의한 영향을 끼치지 않았고, 치주염유발 염증성 사이토카인인인 IL-8과 IL-6의 분비를 억제하였다.



이 논문은 부산대학교 기본연구지원사업(2년)에 의하여 연구되었음.


  1. Park SR, Hong JW, Lee HJ, Kim GC. Plasma Medicine; How can Nonthermal Atmospheric Plasma be Applied to Medicine? J Life Sc 2013;23:838-46.
  2. Kim CH. New conversing technology; plasma medicine. Korean J Otorhinolaryngol-Head Neck Surg 2010;53:593-602.
  3. VON Woedtke T, Schmidt A, Bekeschus S, Wende K, Weltmann KD. Plasma Medicine: A Field of Applied Redox Biology. In Vivo 2019;33:1011-26.
  4. Kim YM, Lee HY, Lee HJ, Kim JB, Kim S, Joo JY, Kim GC. Retention Improvement in Fluoride Application with Cold Atmospheric Plasma. J Dent Res 2018;97:179-83.
  5. Nam SH, Lee HJ, Hong JW, Kim GC. Efficacy of nonthermal atmospheric pressure plasma for tooth bleaching. ScientificWorldJournal 2015;2015:581731.
  6. Armand A, Khani M, Asnaashari M, AliAhmadi A, Shokri B. Comparison study of root canal disinfection by cold plasma jet and photodynamic therapy. Photodiagnosis Photodyn Ther 2019;26:327-33.
  7. Pan J, Sun K, Liang Y, Sun P, Yang X, Wang J, Zhang J, Zhu W, Fang J, Becker KH. Cold plasma therapy of a tooth root canal infected with enterococcus faecalis biofilms in vitro. J Endod 2013;39:105-10.
  8. Lee CM, Jeong YI, Kook MS, Kim BH. Combinatorial Effect of Cold Atmosphere Plasma (CAP) and the Anticancer Drug Cisplatin on Oral Squamous Cell Cancer Therapy. Int J Mol Sci 2020;21:7646.
  9. Heitz-Mayfield LJ, Trombelli L, Heitz F, Needleman I, Moles D. A systematic review of the effect of surgical debridement vs non-surgical debridement for the treatment of chronic periodontitis. J Clin Periodontol 2002;29 Suppl 3:92-102.
  10. Bartold PM, Van Dyke TE. Host modulation: controlling the inflammation to control the infection. Periodontol 2000 2017;75:317-29.
  11. Schueller K, Hans J, Pfeiffer S, Walker J, Ley JP, Somoza V. Identification of Interleukin-8-Reducing Lead Compounds Based on SAR Studies on Dihydrochalcone-Related Compounds in Human Gingival Fibroblasts (HGF-1 cells) In Vitro. Molecules 2020;25:1382.
  12. Eaton KA, Kaiser JB, Davies RM. The removal of root surface deposits. J Clin Periodontol 1985;12:141-52.
  13. Cobb CM. Lasers and the treatment of periodontitis: the essence and the noise. Periodontol 2000 2017;75:205-95.
  14. Park EJ, Kwon EY, Kim HJ, Lee JY, Choi J, Joo JY. Clinical and microbiological effects of the supplementary use of an erythritol powder air-polishing device in non-surgical periodontal therapy: a randomized clinical trial. J Periodontal Implant Sci 2018;48:295-304.
  15. Mahasneh A, Darby M, Tolle SL, Hynes W, Laroussi M, Karakas E. Inactivation of Porphyromonas gingivalis by low-temperature atmospheric pressure plasma. Plasma Med 2011;1:191-204.
  16. Shimada Y, Komatsu Y, Ikezawa-Suzuki I, Tai H, Sugita N, Yoshie H. The effect of periodontal treatment on serum leptin, interleukin-6, and Creactive protein. J Periodontol 2010;81:1118-23.
  17. Lagdive SS, Marawar PP, Byakod G, Lagdive SB. Evaluation and comparison of interleukin-8 (IL-8) level in gingival crevicular fluid in health and severity of periodontal disease: a clinico-biochemical study. Indian J Dent Res 2013;24:188-92.
  18. Begley CG, Ellis LM. Drug development: Raise standards for preclinical cancer research. Nature 2012;28:531-3.
  19. Wei M, Zhang R, Zhang F, Zhang Y, Li G, Miao R, Shao S. An Evaluation Approach of Cell Viability Based on Cell Detachment Assay in a Single-Channel Integrated Microfluidic Chip. ACS Sens 2019;4:2654-61.
  20. Hegde R, Awan KH. Effects of periodontal disease on systemic health. Dis Mon 2019;65:185-92.
  21. Takashiba S, Naruishi K, Murayama Y. Perspective of cytokine regulation for periodontal treatment: Fibroblast biology. J Periodontol 2003;74:103-10.
  22. Finoti LS, Nepomuceno R, Pigossi SC, Corbi SC, Secolin R, Scarel-Caminaga RM. Association between interleukin-8 levels and chronic periodontal disease: A PRISMA-compliant systematic review and meta-analysis. Medicine (Baltimore) 2017;96:e6932.
  23. Naruishi K, Nagata T. Biological effects of interleukin-6 on Gingival Fibroblasts: Cytokine regulation in periodontitis. J Cell Physiol 2018;233:6393-400.
  24. Shindo S, Hosokawa Y, Hosokawa I, Shiba H. Interleukin (IL)-35 Suppresses IL-6 and IL-8 Production in IL-17A-Stimulated Human Periodontal Ligament Cells. Inflammation 2019;42:835-40.
  25. Xiong G, Ji W, Wang F, Zhang F, Xue R, Cheng M, Sun Y, Wang X, Zhang T. Quercetin Inhibits Inflammatory Response Induced by LPS from Porphyromonas gingivalis in Human Gingival Fibroblasts via Suppressing NF-κB Signaling Pathway. Biomed Res Int 2019;2019:6282635.
  26. Muniz FW, Nogueira SB, Mendes FL, Rosing CK, Moreira MM, de Andrade GM, de Sousa Carvalho R. The impact of antioxidant agents complimentary to periodontal therapy on oxidative stress and periodontal outcomes: A systematic review. Arch Oral Biol 2015;60:1203-14.
  27. Kleineidam B, Nokhbehsaim M, Deschner J, Wahl G. Effect of cold plasma on periodontal wound healingan in vitro study. Clin Oral Investig 2019;23:1941-50.
  28. Zhang Y, Xiong Y, Xie P, Ao X, Zheng Z, Dong X, Li H, Yu Q, Zhu Z, Chen M, Chen W. Non-thermal plasma reduces periodontitis-induced alveolar bone loss in rats. Biochem Biophys Res Commun 2018;503:2040-6.
  29. Soares DJ, Walker J, Pignitter M, Walker JM, Imboeck JM, Ehrnhoefer-Ressler MM, Brasil IM, Somoza V. Pitanga (Eugenia uniflora L.) fruit juice and two major constituents thereof exhibit anti-inflammatory properties in human gingival and oral gum epithelial cells. Food Funct 2014;5:2981-8.
  30. Hajishengallis G, Chavakis T, Lambris JD. Current understanding of periodontal disease pathogenesis and targets for host-modulation therapy. Periodontol 2000 2020;84:14-34.