The Korean Journal of Pharmacology (대한약리학회지)

The Korean Society of Pharmacology (대한약리학회)
권호 표지

The effect of Aconiti tuber butanol fraction on the rabbit heart microsomal $Na^+-K^+$-activated ATPase activity

부자 Butanol Fraction이 가토 심장근 Microsomal $Na^+-K^+$-activated ATPase 활성도에 미치는 영향

  • Shin, S.G. (Department of Pharmacology, College of Medicine, Seoul National University) ;
  • Lim, J.K. (Department of Pharmacology, College of Medicine, Seoul National University) ;
  • Park, C.W. (Department of Pharmacology, College of Medicine, Seoul National University) ;
  • Kim, M.S. (Department of Pharmacology, College of Medicine, Seoul National University)
  • 신상구 (서울대학교 의과대학 약리학교실) ;
  • 임정규 (서울대학교 의과대학 약리학교실) ;
  • 박찬웅 (서울대학교 의과대학 약리학교실) ;
  • 김명석 (서울대학교 의과대학 약리학교실)
  • Published : 1976.06.30
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Abstract

Aconiti tuber butanol fraction shows positive inotropic effect on the isolated atrium of rabbit heart. To investigate the mechanism, the effect on microsomal ATPase activity of rabbit heart is observed. The microsomal fraction which contains the $Na^+$- and $K^+$-activated ATPase in the presence of $Mg^{++}$ is isolated from the left ventricle of rabbit heart. The microsomal ATPase activity is maximally stimulated at $Na^+$ and $K^+$ concentration of 100 mM and 10 mM respectively. Microsomal $Na^+-K^+$-activated ATPase is inhibited by ouabain and Aconiti tuber butanol fraction. Ouabain and Aconiti tuber butanol fraction depress $Na^+$-stimulation on microsomal ATPase activity, and the inhibitory effects are not completely reversed at $Na^+$ concentration of 300 mM. Also, $K^+$-stimulation on microsomal ATPase activity is inhibited by ouabin and Aconiti tuber butanol fraction and the inhibitions are not compeletely reversed at $K^+$ concentration of 30 mM. It is, therefore, suggested that the inhibitory effect of Aconiti tuber butanol fraction on the microsomal ATPase activity may contribute to leading to the positive inotropic effect.

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