Chlorella배양(培養)에 있어서 $N^{15}$-표식(標識)와 생육경로(生育經路)에 관한 연구(硏究)

A Study on the $N^{15}-labelling$ and Path Way of Chlorella in the Course of Culture

Chlorella protein의 장기내(臟器內)로의 흡수상태(吸收狀態) 및 그 분포(分布) 등(等)을 알려면 chlorella protein을 동위원소(同位元素)로 표시(標識)해야 된다는 점에 착안(着眼)하여 chlorella를 생육(生育)할 때 $N^{15}$를 배양액(培養液)에다 첨가(添加)하여 $N^{15}$ 표식(標識)된 chlorella protein을 얻을 수 있는가를 검사(檢討)하였다. 배양액내(培養液內)의 N 및 $N^{15}$의 함량(含量)은 배양기간중(培養基間中) 경시적(經時的)으로 증가(增加)하였으나 조도(照度)를 없앤 어두운 상태에서는 증가(增加)하지 않았으며, N 및 $N^{15}$의 흡수상태(吸收狀熊)는 거의 평행(平行)인 것으로 보아 chlorella 막체내(漠體內)에 $N^{15}$가 흡수(吸收)되고 chlorella protein이 $N^{15}$로 표시(標識)된다는 것을 알 수 있었다.

Since chlorella was found to be a source of protein in 1974, wide ranges of investigations on culture methods, its constituents and nutritional factors have been carried out, i. e. most of them were the reports on the nutritional evaluation. However, kinetics such as absorption, distribution, metabolism and elimination of chlorella protein have not been fully elucidated. So, on the assumption that using $N^{15}$ labelled chlorella protein could accomplish good results for kinetics of chlorella in in vivo experiments, $N^{15}$ was added to the culture fluid. From the result of this study, it is suggested that chlorella utilizes N as well as $N^{15}$ in protein synthesis, and this $N^{15}$ labelled chlorella protein can be useful tool for the study of kinetics of chlorella in in vivo experiments.