Isolation and Characterization of Rhizobia from Soybean Cultivated in Korea

대두(大豆) 근류균(根瘤菌)의 분리(分離) 및 특성(特性)

Soybean rhizobia were isolated from 101 soybean (Glycine max.) cultivar which had been grown for the breeding experiment in Korea. Seven strains of the fast-growing soybean rhizobia and nine strains of the slow-growing soybean rhizobia were selected on the basis of their growth rate in AMA medium and their high ability of nodulation. The slow-growing soybean rhizobia were identified as Bradyrhizobium japonicum in the acetylene-reducing activity, microbial characteristics, and biochemical characteristics whereas the fast-growing soybean rhizobia were very similar to Rhizobium fredii.

한국에서 시험재배 되고 있는 대두(Glycine max.) 101품종의 근류로 부터 근류균을 분리하여 AMA 고체배지 상에서의 생육속도 및 근류형성력에 따라 fast-growing soybean rhizobia 7 균주와 slow-growing soybean rhizobia 9균주를 선발하였다. 선정균의 질소고정력, 미생물학적 특징 및 생화학적 특징을 조사한 결과 slow-growing soybean rhizobia 는 .Bradyrhizobium japonicum 과 일치하였으며, fast-growing soybean rhizobia는 Rhizobium fredii 와 유사하였다.