Applied Biological Chemistry
- Volume 30 Issue 3
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- Pages.234-241
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- 1987
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- 2468-0834(pISSN)
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- 2468-0842(eISSN)
Enzymatic Modification of Sardine Protein Concentrate
정어리 분말(粉末) 단백질(蛋白質)의 효소적(酵素的) 수식(修飾)
- Kim, Se-Kwon (Department of Applied Chemistry, National Fisheries University of Pusan) ;
- Lee, Eung-Ho (Department of Food Science and Technology, National Fisheries University of Pusan)
- Published : 1987.09.30
Abstract
Conditions necessary for optimal plastein productivity from sardine protein hydrolysate using papain and pepsin were established. Sardine protein concentrate was hydrolyzed with pepsin yielding an approximate degree of hydrolysis of 77.2%. Enzyme induced plastein was optimized at: pH 6 for papain and pH 4 for pepsin; substrate concentrate, 50%(w/v) for papain and 40%(w/v) for pepsin; time of incubation, 24hr; enzyme/substrate ratio, 1 : 100(w/w). Plastein yields of 49.5% and 45.3% were found for papain and pepsin, respectively, when 10% trichloroacetic acid (TCA) was used as the precipitating agent. However, when plastein was precipitated by 50% ethanol, the yield was found to be 43.6% and 41.0% for papain and pepsin, respectively. Ethanol-precipitated plastein did not contain lipid and contained approximately 1.3% ash and 91.0% protein. In comparison, the TCA-precipitated plastein contained 74.2% protein, 0.5% lipid and 15.3% ash.
어육(魚肉) 단백질(蛋白質)의 기능성(機能性)을 개선(改善)하기 위해 정어리 분말단백질(粉末蛋白質)의 pepsin 가수분해물(加水分解物)을 이용(利用)하여 plastein을 합성(合成)하기 위한 반응조건(反應條件)을 검토(檢討)하였다. Plastein의 합성조건(合成條件)은 papain 및 pepsin의 경우 pH는 각각(各各) 6, 4, 기질농도(基質濃度)는 각각(各各) 50%, 40%, 반응시간(反應時間) 및 효소농도(酵素濃度)는 각각(各各) 2시간(時間), 1%였으며 반응온도(反應溫度)는 papain 및 pepsin 모두
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