Toxicological Research
- Volume 4 Issue 2
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- Pages.117-129
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- 1988
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- 1976-8257(pISSN)
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- 2234-2753(eISSN)
QUANTITATION OF OXANDROLONE(A SYNTHETIC ANABOLIC STEROID) IN HUMAN URINE BY GC/MS
- Park, Jongsei (Dopin Control Center, Korean Advanced Institute of Science and Technology) ;
- Ohseung Kwon (Dopin Control Center, Korean Advanced Institute of Science and Technology) ;
- Hea-Young P. Choo (Dopin Control Center, Korean Advanced Institute of Science and Technology) ;
- Jawon Suh (Dopin Control Center, Korean Advanced Institute of Science and Technology)
- Published : 1988.12.01
Abstract
A sensitive method for the quantitation of oxandrolone in urine was developed using GC/MS. After oral administration of 10mg oxandrolone, oxandrolone excreted in urine as unchanged form was extracted in ether and derivatized to its O-TMS. Oxandrolone excreted in urine as glucuronide conjugated form was extracted after enzymatic hydrolysis and derivatized to its O-TMS. The amounts of oxandrolone-O-TMS was measured in GC/MS with selected ion monitoring. Calusterone, a structurally similar anabolic steroid, was employed as internal standard.
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