Toxicological Research
- Volume 4 Issue 2
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- Pages.143-149
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- 1988
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- 1976-8257(pISSN)
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- 2234-2753(eISSN)
AN EVIDENCE FOR THE INVOLVEMENT OF CYTOLYSIN IN VIBRIO VULNIFICUS DISEASE
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Park, Moon-Kook
(Department of Biological Education, College of Education, Chonbuk National University)
- Published : 1988.12.01
Abstract
Cytolysin produced by Vibrio vulnificus ATCC 27562 was partially purified by sequential ammonium sulfate precipitation, gel filtration with Sephadex G-200, and ion exchange chromatography with DEAE-Sephadex. The partially purified cytolysin was inactivated by cholesterol. More than one molecule of the cytolysin was required to lyse a single erythrocyts. The antiserum against cytolysin enhanced the survival ratio of mice infected with low dose of V. vulnificus.