Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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CO-CULTURE OF BOVINE EMBRYOS WITH CUMULUS CELLS

  • Goto, K. (Laboratory of Animal Reproduction, Department of Animal Science, Faculty of Agriculture, Kagoshima University) ;
  • Koba, M. (Laboratory of Animal Reproduction, Department of Animal Science, Faculty of Agriculture, Kagoshima University) ;
  • Takuma, Y. (Laboratory of Animal Reproduction, Department of Animal Science, Faculty of Agriculture, Kagoshima University) ;
  • Nakanishi, Y. (Laboratory of Animal Reproduction, Department of Animal Science, Faculty of Agriculture, Kagoshima University) ;
  • Ogawa, K. (Laboratory of Animal Reproduction, Department of Animal Science, Faculty of Agriculture, Kagoshima University)
  • Received : 1989.06.27
  • Accepted : 1989.09.20
  • Published : 1989.12.01
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Abstract

Bovine embryos/ova obtained from in-vitro fertilization were either co-cultured on a monolayer of bovine cumulus cells or cultured in medium alone. Embryos/ova co-cultured with cumulus cells developed to 8-cell (30.9%), morula (29.8%) and blastocyst stages (26.6%) after 3-4, 5-6, and 7-8 days of culture, respectively, while embryos/ova cultured in medium alone failed to develop beyond 8-cell (0-13.3%), morula (0-1.5%) and blastocyst stages (0%). The results of this study demonstrated the beneficial effect of cumulus cells on the development of bovine embryos.

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