Development of Mouse Embryos in Vitro and Vivo by Quick-splitting

양분(兩分)한 생쥐 배(胚)의 체외(體外) 및 체내(體內) 발생(發生)에 관한 연구(硏究)

The study was carried out to investigate the viability of mouse embryos bisected at the stages of 8-cell, morula and blastocyst and, also, to find out the feasibility of offspring production by transfer of the bisected blastocysts with Quick-splitting. The results obtained are summarized as follows: 1. The mouse embryos were bisected with bio-cut blade at the stages of 8-cell and morula, and cultured in Medium 2. The bisected embryos were developed to blastocyst stage by 64% and 81%. respectively. 2. The blastocyts which were cultured in Medium 2 after being bisected at the stages of 8-cell and morula were observed normal outgrowth in Ham's F-10 medium by 86% and 90%, respectively. 3. The blastocyts which were Quick-splitted in Medium 2 were observed normal outgrowth by 97%. however, no offspring was obtained by transfer of Quick-splitted blastocysts.

본(本) 연구는 micromanipultor를 이용하여 생쥐의 8세포기배와 상실배 그리고 배반포기배를 양분(兩分)후 생존성을 검토하고, 또한 배반포기배를 양분후, 선별(選別)및 배양 과정없이 암컷 생쥐에 이식(移植)하는 경우 새끼쥐 생산의 가능성을 검토하고자 수행된 것이다. 그 경과를 요약해 보면 다음과 같다. 1. 생쥐의 8세포기배(細胞期胚)와 상실배(桑實胚)를 양분(兩分)하여 M2에 배양한결과 각각 64%, 81%가 배반포기배(胚盤胞期胚)까지 발생(發生)하였다. 2. M2 배양액에서 발생시킨 배반포기배를 Ham's F-10에서 배양한 결과 8세포기배에서는 86%, 상실배에서는 90%가 정상적으로 outgrowth 되었다. 3. 배반포기배를 배양 과정없이 바로 양분(兩分)하여 Ham's F-10에서 배양한 결과 97%가 정상적으로 outgrowth 되었으나 암컷생쥐에 이식한 결과 산차는 얻지 못하였다.