The Korean Journal of Pharmacology (대한약리학회지)

The Korean Society of Pharmacology (대한약리학회)
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Effects of Ethanol on the Fluidity of Plasma Membrane Vesicles Isolated from Cultured Mouse Myeloma Cell Line Sp2/0-Ag14

Ethanol이 배양된 Mouse Myeloma Cell Line Sp2/0-Ag14로부터 분리한 형질막의 유동성에 미치는 영향

  • Yun, Il (Department of Dental Pharmacology, Research Institute for Oral Biotechnology, Pusan National University) ;
  • Chung, In-Kyo (Department of Biophysics, Research Institute for Oral Biotechnology, Pusan National University) ;
  • Park, Yeong-Min (Department of Biophysics, Research Institute for Oral Biotechnology, Pusan National University) ;
  • Kim, Jin-Bom (Department of Preventive Dentistry, Research Institute for Oral Biotechnology, Pusan National University) ;
  • Yu, Seoung-Ho (Department of Oral Microbiology, Research Institute for Oral Biotechnology, Pusan National University) ;
  • Kang, Jung-Sook (Department of Oral Biochemical and Molecular Biology, Research Institute for Oral Biotechnology, Pusan National University)
  • 윤일 (부산대학교 구강생물공학연구소) ;
  • 정인교 (부산대학교 구강생물공학연구소) ;
  • 박영민 (부산대학교 구강생물공학연구소) ;
  • 김진범 (부산대학교 구강생물공학연구소) ;
  • 유성호 (부산대학교 구강생물공학연구소) ;
  • 강정숙 (부산대학교 구강생물공학연구소)
  • Published : 1993.06.30
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Abstract

Intramolecular excimerization of 1,3-di(1-pyrenyl)propane (Py-3-Py) and fluorescence polarization of 1,6-diphenyl-1,3,5-hexatriene (DPH) were used to examine the effects of ethanol on the rate and range of lateral diffusion of bulk bilayer structures of plasma membrane vesicles isolated from cultured mouse myeloma cell line Sp2/0-Ag14 (Sp2/0-PMV). In a concentration-dependent manner, ethanol increased the excimer to monomer fluorescence intensity ratio (I'/I) of Py-3-Py in the Sp2/0-PMV and decreased the anisotropy (r), limiting anisotropy $(r_{\infty})$, and order parameter (S) of DPH in the Sp2/0-PMV. This indicates that ethanol increased both the lateral and rotational diffusion of the probes in the Sp2/0-PMV. Selective quenching of DPH by trinitrophenyl groups was utilized to examine the transbilayer asymmetric rotational diffusion of the Sp2/0-PMV. The anisotropy (r), limiting anisotropy $(r_{\infty})$, and order parameter (S) of DPH in the inner monolayer were 0.022, 0.029, and 0.063, respectively, greater than calculated for the outer monolayer of the Sp2/0-PMV. Selective quenching of DPH by trinitrophenyl groups was also utilized to examine the transbilayer asymmetric effects of ethanol on the range of rotational diffusion of the Sp2/0-PMV. Ethanol had a greater fluidizing effect on the outer monolayer as compared to the inner monolayer of the Sp2/0-PMV. It has been proven that ethanol exhibits a selective rather than nonselective fluidizing effect within transbilayer domains of the Sp2/0-PMV.

Ethanol이 암세포 증식 속도에 미치는 직접적인 영향 검색의 일환으로 배양된 mouse myeloma cell line Sp2/0-Ag14로부터 분리한 형질막 (Sp2/0-PMV) 유동성에 미치는 ethanol의 영향을 형광분석법으로 측정하였다. 그 결과 ethanol은 Sp2/0-PMV 지질 이중층 측방확산운동의 범위와 속도를 증가시켰고 회전확산운동 범위도 증가시켰다. 특히 ethanol은 Sp2/0-PMV 지질이중층 중 내부단층 (inner monolayer)에 비하여 비교적 선택적으로 외부단층 (outer monolayer)의 회전확산운동 범위를 증가시킨다는 것을 확인하였다.

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