INFLUENCE OF MEMBRANE LIPIDS ON RETINAL-AMINE INTERACTON

  • Singh, Anil K. (Department of Chemistry, Indian Institute of Technology) ;
  • Das, Joydip (Department of Chemistry, Indian Institute of Technology)
  • Published : 1996.05.01

Abstract

The effect of vesicular L-$\alpha$-phosphatidylcholine on the rate of formation of all-trans-N-retinylidene-n-butylamine (3) and on the regeneration kinetics of bacteriorhodopsin pigment from retinal and bacterio-opsin have been studied. An estimate of the relative positions of retinal and n-butylamine in the vesicles has been made by fluoresence quenching experiments. Partition coefficient of retinal and microviscosity of the retinal-binding region have also been determined. The results are discussed in terms of the nature of chemical interaction between retinal and amine in a lipid environment.

Keywords

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