The Korean Journal of Physiology and Pharmacology

The Korean Society of Pharmacology (대한약리학회)
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Inhibition by Higenamine of Lipopolysaccharide-induced iNOS mRNA Expression and NO Production in Rat Aorta

Lipopolysaccharide로 활성화시킨 흰쥐 혈관의 iNOS 발현에 대한 Higenamine의 효과

  • Kang Young-Jin (Department of Pharmacology College of Medicine, Gyeongsang National University) ;
  • Lee Goun-Woo (Department of Pharmacology College of Medicine, Gyeongsang National University) ;
  • Ku Eui-Bon (Department of Pharmacology College of Medicine, Gyeongsang National University) ;
  • Lee Hoi-Young (Department of Pharmacology College of Medicine, Gyeongsang National University) ;
  • Chang Ki-Churl (Department of Pharmacology College of Medicine, Gyeongsang National University)
  • 강영진 (경상대학교 의과대학 약리학교실) ;
  • 이균우 (경상대학교 의과대학 약리학교실) ;
  • 구의본 (경상대학교 의과대학 약리학교실) ;
  • 이회영 (경상대학교 의과대학 약리학교실) ;
  • 장기철 (경상대학교 의과대학 약리학교실)
  • Published : 1997.06.01
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Abstract

Higenamine was widely used as traditional remedy for the treatment of rhumatoid arthritis. Nitric oxide(NO) may be a critical mediator in this inflammatory disease. Synovial tissue from humans with inflammatory arthritis expresses NOS2(iNOS) mRNA and protein, and generates NO in vitro. We therefore, investigated the effect of higenamine on the induction of nitric oxide synthase(NOS) promoted by lipopolysaccharide(LPS). Prophylactic application of higenamine selectively prevented LPS-primed initiation of L-arginine-induced relaxation and restored rhenylephrine(PE)-induced contraction in rat aorta. LPS-stimulated nitrite production in the incubation medium was reduced by higenamine. Furthermore, RT-PCR and Northern analysis indicated that higenamine reduced iNOS expression primed by LPS in rat aorta. These results suggest that higenamine prevents LPS-promoted induction of NOS in vascular smooth muscle.

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