Animal cells and systems

The Korean Society for Integrative Biology (한국통합생물학회)
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Determination of HLA-A*02 Alleles Using Nested PCR-SSP in Korean Population

  • Lee, Kyung-Ok (Department of Moecular Genetics, Seoul Medical Science institute) ;
  • Heo, Jeong-Ho-Ho (Department of Moecular Genetics, Seoul Medical Science institute) ;
  • Kim, Hye-Jin (Immunology Laboratory, Severance Hospital, Yonsei University) ;
  • Lee, Eun-Mi (Immunology Laboratory, Severance Hospital, Yonsei University) ;
  • Hong, Sung-Hoi (Department of Moecular Genetics, Seoul Medical Science institute) ;
  • Kim, Yoon-Jung (Department of Moecular Genetics, Seoul Medical Science institute)
  • Published : 1997.03.01
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Abstract

HLA-A2 is one of the most diversified HLA-class I antigen with 17 subtypes so far identified at the molecular level. HLA-A*02 subtyping has significant implications on the tissue typing for organ and bone marrow transplantations. Recently, DNA-based typing methods have been successfully applied to the elucidation of HLA gene polymorphisms. In the present study, HLA-A*O2 genotyping was established by using nested polymerase chain reaction-sequence specific primers (PCR-SSP) and distribution of A*O2 alleles were determined in Korean individuals. Genomic DNA prepared from four B-lymphoblastoid cell lines and lymphocytes from serologically defined 48 HLA-A2 Korean individuals by phenol/chloroform extractions was typed. The results of the four B-lymphoblastoid cells were consistent with the previous data typed by PCR analysis. Five A*O2 alleles-A*0201, A*0203, A*0206, A*0207 and A*0210-were commonly observed in a total of 17 A*02 alleles. Of these, A*0207 (f=49.0%) was the most frequent allele in Korean population. A*0206 (f=28.3%) and A*0201 (f=17.0%) were also found frequently while A*0203 and A*0210 types were observed in less than 5%. In conclusion, the high level of discrimination for HLA-A*O2 alleles will prove useful and informative in the study of transplant survival, and may identify the importance of allelic differences not readily detectable by serology on host and donor compatibility.

Keywords

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