Animal cells and systems

The Korean Society for Integrative Biology (한국통합생물학회)
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Analysis for Regulatory Elements in Yeast MGMT Gene Transcription

  • Joo, Jae-Hoon (Department of Molecular Biology and Research Center for Cell Differentiation, Seoul National University) ;
  • Kim, Woo-Jae (Department of Molecular Biology and Research Center for Cell Differentiation, Seoul National University) ;
  • Rho, Jae-Kyun (Department of Biology, Chungbuk National University) ;
  • Choe, Jae-Hyun (Department of Biology, Chungbuk National University) ;
  • Choe, Soo-Young (Department of Biology, Chungbuk National University) ;
  • Sang-Dai (Department of Molecular Biology and Research Center for Cell Differentiation, Seoul National University)
  • Published : 1998.06.01
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Abstract

The Saccharomyces cerevisiae MGMT gene encodes a O6-methylguanine DNA methyltransferase that protects cells from mutation or death by DNA alkylating agents. Using an in vitro transcription system, we analyzed its promoter region to find regulatory elements for transcription initiation. DNase I footprinting and a transcription assay showed that a functional TATA box, 5'-TGATATAGCA-3', is located in the region spanning from -25 to -34. We also found one upstream repressing sequence (URS), -333 to -213, by promoter deletion and competition analysis. Gel mobility shift assays and Southwestern blot analysis using URS region indicate specific complex formations. These results indicate that several cis-acting and trans-acting elements might be involved in the transcriptional regulation of the S. cerevisiae MGMT gene.

Keywords

References

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