Animal cells and systems

The Korean Society for Integrative Biology (한국통합생물학회)
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Identification and Characterization of a RecA-like Protein Induced by DNA Damaging Agents in Fluorescent Pseudomonas sp.

  • Kim, Ok-Bong (Department of Biological Science, Chosun University) ;
  • Lim, Chae-Kwang (Department of Biological Science, Chosun University) ;
  • Kim, Si-Wouk (Department of Environmental Engineering, Chosun University) ;
  • Park, Jong-Kun (Division of life Science and Medicinal Resources Research Center) ;
  • Yoon, Seong-Myeong (Department of Biology Education, chosun University) ;
  • Lee, Jung-Sup (Department of Biological Science, Chosun University)
  • Published : 1998.09.01
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Abstract

A RecA-like protein (RecAps) was identified from fluorescent Pseudomonas sp. and the inducible nature of the protein was characterized in detail. It was shown by dose-response and time-course experiments using two DNA damaging agents, nalidixic acid and mitomycin-C, that the cellular level of RecAps protein was increased 3-8 fold compared to that of the control. The most effective doses of nalidixic acid and mitomycin-C for the protein induction were $30{\mu}g/ml$ and $0.3{\mu}g/ml$ at the treatment time point of 150 min, respectively. The enhanced level of RecAps protein was gradually decreased to the control level after 10 hr in normal medium. Interestingly, the cellular level of RecAps protein was increased by the same DNA damaging agents even when cell growth was completely inhibited by treatment with $170{\mu}g/ml$ of chloramphenicol, an inhibitor of protein synthesis, suggesting that new protein synthesis is not required for the induction of RecAps. All these results suggest that a typical S0S repair function driven by RecA-like protein is conserved in Pseudomonas sp. cells as in E, coli.

Keywords

References

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