Animal cells and systems

The Korean Society for Integrative Biology (한국통합생물학회)
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Effects of Retinoic Acid and cAMP on the Differentiation of Naegleria gruberi Amoebas into Flagellates

  • Bora Kim (Department of Biology, College of Science, Yonsei University) ;
  • Hong Kyoung Kim (Department of Biology, College of Science, Yonsei University) ;
  • Daemyoung Kim (Department of Genetic Engineering, Chungjoo University) ;
  • In Kwon Chung (Department of Biology, College of Science, Yonsei University) ;
  • Young Min Kim (Department of Biology, College of Science, Yonsei University) ;
  • Jin Won Cho (Department of Biology, College of Science, Yonsei University) ;
  • JooHun Lee (Department of Biology, College of Science, Yonsei University)
  • Published : 1999.06.01
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Abstract

During the differentiation of Naegleria gruberi amoebas into flagellates, the amoebas undergo sequential changes in cell shape and form new cellular organelles. To understand the nature of the signal which initiates this differentiation and the signal transduction pathway, we treated cells with four agents, PMA, retinoic acid (RA), okadaic acid, and cAMP. Retinoic acid and cAMP had specific effects on the differentiation of N. gruberi depending on the time of the drug treatment. Addition of (100$\mu$M) retinoic acid at the initiation of differentiation inhibited differentiation by blockinq the transcription of differentiation specific genes (e.g., $\beta$-tubulin). This inhibition of differentiation by retinoic acid was overcome by co-treatment with cAMP (or dbcAMP, 20 $\mu$M). Addition of retinoic acid at later stages (30 and 70 min) had no effect on the transcriptional regulation of the $\beta$-tubulin gene, however the differentiation was inhibited by different degrees. Co-treatment of cAMP at these stages did not overcome the inhibitory effect of retinoic acid. These results suggest that the role of retinoic acid as a transcriptional regulator might be conserved throughout the evolution of eukaryotes.

Keywords

References

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