Journal of Ginseng Research
- Volume 23 Issue 1 Serial No. 53
- /
- Pages.55-59
- /
- 1999
- /
- 1226-8453(pISSN)
- /
- 2093-4947(eISSN)
A Simple Mehtod for Conjugation Between Ginsenoside Rf and Bovine Serum Albumin
- Yoon So-Rah (Department of Physiology, College of Veterinary Medicine Chonnam National University) ;
- Nah Jin-Ju (Department of Physiology, College of Veterinary Medicine Chonnam National University) ;
- Choi Seok (Department of Physiology, College of Veterinary Medicine Chonnam National University) ;
- Son Chang-Ho (Department of Physiology, College of Veterinary Medicine Chonnam National University) ;
- Shin Dong-Ho (Department of Physiology, College of Veterinary Medicine Chonnam National University) ;
- Ko Sung-Ryong (Korea Ginseng and Tobacco Research Institute) ;
- Kim Seok-Chang (Korea Ginseng and Tobacco Research Institute) ;
- Nam Ki-Yeul (Korea Ginseng and Tobacco Research Institute) ;
- Nah Seung-Yeol (Korea Ginseng and Tobacco Research Institute)
- Published : 1999.03.01
Abstract
For the first time we have developed a simple method for conjugation between ginsenoside Rf (Rf) and bovine serum albumin (BSA) or ovalbumin (OVA) by a periodate oxidation method. The Rf-BSA conjugate is confirmed by ultraviolate (UV) scanning, thin layer chromatography (TLC), and SDS-gel electrophoresis. In UV scanning Rf showed three small peaks approximately at 230, 265 and 280 nm. BSA showed a peak at 280 nm. The Rf-BSA conjugate showed right shifted three peaks that BSA alone did not show. In TLC analysis, the Rf-BSA conjugate did not show mobility in silica gel but showed a slight stream of trace. In SDS-gel electrophoresis, Rf-BSA conjugate show a slight less mobility than BSA alone. Rf-OVA conjugate also showed similar patterns with Rf-BSA conjugate. These results demonstrated that periodate oxidation method could be used to produce a stable Rf-BSA or Rf-OV A conjugate and also could be applied for other ginsenoside(s).