The Journal of Internal Korean Medicine (대한한방내과학회지)

The Society of Internal Korean Medicine (대한한방내과학회)
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Effects of Jagamcho-tang on the C6 Glial Cell Injured by LPS Combined PMA

자감초탕(炙甘草湯)이 LPS와 PMA에 의해 손상된 C6 glial 세포에 미치는 영향

  • Cho, Nam-Su (Dept. of Internal Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Rhyu, Jun-Ki (Dept. of Internal Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Lee, In (Dept. of Internal Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Shin, Sun-Ho (Dept. of Internal Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Moon, Byung-Soon (Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Na, Young-Hoon (Professional Graduate School of Oriental Medicine, Wonkwang University)
  • 조남수 (원광대학교 한의과대학 심계내과학교실) ;
  • 유준기 (원광대학교 한의과대학 심계내과학교실) ;
  • 이인 (원광대학교 한의과대학 심계내과학교실) ;
  • 신선호 (원광대학교 한의과대학 심계내과학교실) ;
  • 문병순 (원광대학교 한의학전문대학원) ;
  • 나영훈 (원광대학교 한의학전문대학원)
  • Published : 2000.11.30
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Abstract

The water extracts of Jagamcho-tang has been used for treatment of arrhythmia and palpitation in oriental traditional medicine. Brain is provided with blood flow by heart. Jagamcho-tang has been studied on ischemia and infarction in heart. However, little is known about the mechanism by which the water extracts of Jagamcho-tang rescues brain cells from ischemic damages. To elucidate the protective mechanism on ischemic induced cytotoxicity, the effects of Jagamcho-tang on ischemia induced cytotoxicity and generation of nitric oxide(NO) are investigated in C6 glioma cells. Jagamcho-tang induce NO in a dose dependent manner up to 2.5mg/ml in C6 glioma cells. The pretreatment of Jagamcho-tang protect sodium nitroprusside(SNP) (2mM) induced cytotoxicity. This effect of Jagamcho-tang is mimicked by treatment by pretreatment of SNP($100{\mu}M$), an exogenous NO donor. NG-monomethyl-L-arginine($N^{G}MMA$), a specific inhibitor of nitric oxide synthase (NOS), significantly blocks the protective effects of Jagamcho-tang on cell toxicity by ischemia. In addition, lipopolysaccharide(LPS) and phorhol 12 myristate 13-acetate(PMA) treatment for 72h in C6 glial cells markedly induce NO, but treatment of the cells with the water extracts of Jagamcho-tang decrease nitrite formation in a dose dependent manner. In addition, LPS and PMA treatment for 72h induce severe cell death and LDH release into medium in C6 glial cells. However treatment of the cells with the water extracts of Jagamcho-tang dose not induce significant changes compare to control cells. Furthermore, the protective effects of the water extracts of Jagamcho-tang is mimicked by treatment of $N^{G}MMA$. Taken together, I suggest that the protective effects of the water extracts of Jagamcho-tang against ischemic brain damages may be mediated by regulation of iNOS during ischemic condition.

Keywords

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