Journal of Photoscience

Korean Society of Photoscience (한국광과학회)
권호 표지

Inactivation of Photosystem I in Cucumber Leaves Exposed to Paraquat-Induced Oxidative Stress

  • Published : 2001.03.01
Download PDF
⟨ Previous Next ⟩

Abstract

Cucumber leaves subjected to light chilling stress exhibit a preferential inactivation of photosystem(PS) I relative to PSII, resulting in the photoinhibition of photosynthesis. In light chilled cucumber leaves, Cu/Zn-Superoxide dismutase(SOD) is regarded as a primary target of the light chilling stress and its inactivation is closely related to the increased production of reactive oxygen species. In the present study, we further explored that inactivation of PSI in cucumber leaves is not a light chilling specific, but general to various oxidative stresses. Oxidative stress in cucumber leaves was induced by treatment of methylviologen(MV), a producer of reactive oxygen species in chloroplasts. MV treatment decreased the maximal photosynthetic O$_2$ evolution, resulting in the photoinhibition of photosynthesis. The photoinhibition of photosynthesis was attributable to the decline in PSI functionality determined in vivo by monitoring absorption changes around 820 nm. In addition, MV treatment inactivated both antioxidant enzymes Cu-Zn-superoxide dismutase and ascorbate peroxidase known sensitive to reactive oxygen species. From these results, we suggest that chloroplast antioxidant enzymes are the primary targets of photooxidative stress, followed by subsequent inactivation of PSI.

Keywords

References

  1. Trends in Plant Sci v.6 Impacts of chilling temperatures on photosynthesis in warm-climate plants Allen D.J.;Ort D.R.
  2. Plant Cell Environ v.6 Interaction between light and chilling temperature on the inhibition of photosynthesis in chilling-sensitive plants Powles S.B.;Berry J.A.;Bjorkman O.
  3. Photoihibition of Photosynthesis Photoinhibition induced by low temperatures Krause G.H.;Baker NR(eds.);Bowyer JR(eds.)
  4. Planta v.193 The site of photoinhibition in leaves of Cucumis sativus L. at low temperature is photosystem I, not photosystem II. Terashima I;Funayama S.;Sonoike K.
  5. Photosynth. Res. v.40 Photoinhibition of photosynthesis in chilled potato leaves is not correlated with a loss of photosystem II activity Havaux M.;Davaud A.
  6. J. Plant Res. v.111 Various aspects of inhibition of photosynthesis under light/chilling stress : photoinhibition at chilling temperature versus chilling damage in the light Sonoike K.
  7. Chloroplastic Cu/Zn-SOD is a highly sensitive site in cucumber leaves chilled in the light Choi S.M.;Jeong S.W.;Jeong W.J.;Kwon S.Y.;Chow W.S.;Park Y.I.
  8. Annu. Rev. Plant Physiol. Plant Mol. Biol. v.50 The water-water cycle in chloroplasts : scavenging of active oxygen and dissipation of excess photons Asada K.
  9. Free Radic. Biol. Med. v.23 Use of transgenic plants to study antioxidant defenses Allen R.D.;Webb R.P.;Schake S.A.
  10. Ecophysiology of Photosynthesis Chlfluorescence as a nonintrusive indicator for rapid assessment of in vivo photosynthesis Schreiber U.;Bilger W.;Neubauer C.;Schulze E-D(eds.);Caldwell MM(eds.)
  11. Biochem. Biophys. Acta. v.975 Determination of accurate extinction coefficients and simulataneous equations for assaying chlorophylls a and b extracted with four different solvents: verification of the concentration of chlorophyll standards by atomic absorption spectroscopy Porra R.J.;Thompson W.A.;Kriedemann;P.E.
  12. Anal. Biochem. v.44 Superoxide dismutase:improved assays and an assay applicable to acrylamide gels. Beauchamp. C.;Fridovich I.
  13. Differentiation v.13 Developmental expression and intracellular localization of superoxide dismutase in maize Baum J.A.;Scandalios J.G.
  14. Anal. Biochem. v.212 Detection of ascorbate peroxidase activity in native gels by inhibition of the ascorbate dependent reduction of nitroblue tetrazolium Mittler R.;Zilinskas B.A.
  15. Plant Cell Physiol v.38 Inactivation and degradation of CuZn-SOD by active oxygen species in wheat chloroplasts exposed to photooxidative stress Casano L.M.;Comez L.D.;Lascano H.R.;Gonalez C.A.;Trippi V.S.
  16. Biochim. Biophys. Acta. v.1504 Chloroplastic ascorbate peroxidase is the primary target of methyl viologen-induced photooxidative stress in spinach leaves: its relevance to mondehydroascorbate radical detected with in vivo ESR Mano J.;Ohno C.;Domae Y.;Asada K.
  17. Free Radic. Biol. Med. v.23 The regulation and function of tobacco superoxide dismutase. Van Camp W.;Inze D.;Van Montagu M.
  18. Plant Cell Physiol v.37 Inactivation mechanism of ascorbate peroxidase at low concentrations of ascorbate;hydrogen peroxide decomposes compound I of ascorbate peroxidase Miyake C.;Asada K.