The Korean Journal of Physiology and Pharmacology

  • 제6권1호
  • /
  • Pages.47-55
  • /
  • 2002
  • /
  • 1226-4512(pISSN)
  • /
  • 2093-3827(eISSN)
대한약리학회 (The Korean Society of Pharmacology)
권호 표지

Inwardly Rectifying $K^+$ Currents in Gastric Myocytes of Guinea-pig

  • Jun, Jae-Yeoul (Department of Physiology, College of Medicine, Chosun University) ;
  • Yeum, Cheol-Ho (Department of Physiology, College of Medicine, Chosun University) ;
  • Yoon, Pyung-Jin (Department of Physiology, College of Medicine, Chosun University) ;
  • Jang, In-Youb (Department of Anatomy, College of Medicine, Chosun University) ;
  • Cho, Nam-Soo (Department of Emergency Medicine, College of Medicine, Chosun University) ;
  • Cho, Soo-Hyeong (Department of Emergency Medicine, College of Medicine, Chosun University) ;
  • Kong, In-Deok (Department of Physiology, Wonju Medical School, Yonsei University) ;
  • Kim, Tae-Wan (Department of Physiology and Cell Biology, College of Medicine, Seoul Notional University) ;
  • So, In-Suk (Department of Physiology and Cell Biology, College of Medicine, Seoul Notional University) ;
  • Kim, Ki-Whan (Department of Physiology and Cell Biology, College of Medicine, Seoul Notional University)
  • 발행 : 2002.02.21
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

To identify the presence of inwardly rectifying $K^+$ channels and its characteristics, membrane currents were measured using a whole-cell patch clamp from isolated gastric myocytes of guinea-pig. Change of external $K^+$ concentration from 5 to 90 mM induced an inward current at a holding potential of -80 mV. The high $K^+-induced$ inward current was blocked by $Ba^{2+}$ and $Cs^+,$ but not by glibenclamide. With 90 mM $K^+$ in bath, the $Ba^{2+}-$ and $Cs^+-sensitive$ currents showed strong inward rectification. Ten mM TEA weakly blocked the inward current only at potentials more negative than -50 mV. With 90 mM $K^+$ in bath, hyperpolarizing step pulses from -10 mV induced inward currents, which were inactivated at potentials more negative than -70 mV. Reduction of external $K^+$ to 60 mM decreased the amplitudes of the currents and shifted the reversal potential to more negative potential. The inactivation of inward $K^+$ current at negative clamp voltage was not affected by removing external $Na^+.$ These results suggest that the inwardly rectifying $K^+$ channels may exist in gastric smooth muscle.

키워드

참고문헌

  1. Barros F, Delgado LM, Camino D, Pena P. Characteristics and modulation by thyrotropin-releasing hormone of an inwardly rectiJYing $K^+$ current in patch-perforated Ga, anterior pituitary cells. Pfliigers Arch 422: 31-39, 1992
  2. Bauer CK, Meyerhof W, Schwarz JR. An inward-rectiJYing $K^+$ current in clonal rat pituitary cells and its modulation by thyrotrophin-relasing hormone. J Physiol 429: 169-180, 1990
  3. Benham CD, Bolton TB, Denbigh JS, Lang RJ. Inward rectification in freshly isolated single smooth muscle cells of the rabbit jejunum. J Physiol 383: 461-476, 1987
  4. Bolton TB, Beech DJ. Smooth muscle potassium channels: their electrophysiology and function. In Potassium Channel Modulators. Oxford:Blackwell Scientific Publications. ed. Weston, AH. & Hamilton, T.C. ppI44-180, 1992
  5. Corrette BJ, Bauer CK, Schwarz JR. An inactivating inwardrectiJYing $K^+$ current in prolactin cells from pituitary of lactating rats. J Memb Bioi 150: 185-195, 1996
  6. Difrancesco D. The cardiac hyperpolarizing-activated current, if. Origins and developments. Pro Biophy Mole Bioi 46: 163-183, 1985
  7. Edwards FR, Hirst DS. Inward rectification in submucosal arteriols of guinea-pig ileum. J Physiol 404: 437-454, 1988
  8. Edwards FR, Hirst GDS, Silverg GD. Inward rectification in rat cerebral arterioles: involvement of potassium ions in autoregulation. J Physiol 404: 455-466, 1988 https://doi.org/10.1113/jphysiol.1988.sp017299
  9. Green ME, Edwards G, Kirkup AJ, Miller M, Weston AH. Pharmacological characterization of the inwardly-rectiJYing current in the smooth muscle cells of the rat bladder. Br J Pharmacol 119: 1509-1518, 1996
  10. Hagiwara S, Miyazaki S, Rosenthal NP. Potassium current and the effect of cesium on this current during anomalous rectification of egg cell membrane of a starfish. J Cen Physiol 67: 621-638, 1976
  11. Hamill OP, Marty A, Neher E, Sakmann B, Sigworth FJ. Improved patch clamp techniques for high resolution current recording from cells and cell-free membrane patches. Pflogers Arch 391: 85-100, 1981
  12. Harvey RD, Ten Eick RE. Characterization of the inward-rectifying potassium current in cat ventricular myocytes. J Cen Physiol 91: 593-604, 1988
  13. Hoyer J, Popp R, Meyer J, Galla HJ, Gogelein H. Angiotensin II, vasopressin and GTP[ r-S] inhibit inward-rectiJYing $K^+$ channels in porcine cerebral capillary endothelial cells. J Memb Bioi 123:55-62, 1991
  14. Hu Q, Shi YL. Characterization of an inward-rectiJYing potassium current in NG108-15 neuroblastoma x glioma cells. Pflogers Arch 433: 617-625, 1997
  15. Isenberg G, Klockner U. Calcium tolerant ventricular myocytes prepared by pre-incubation in a 'KB-medium'. PIlogers Arch 424: 6-18, 1982
  16. Ishikawa T, Cook DI. Effects of $K^+$ channel blockers on inwardly and outwardly rectiJYing whole-cell $K^+$ currents in sheep parotid secretory cells. J Memb Bioi 133: 29-41, 1993
  17. Jow F, Numann R. Divalent ion block of inward rectifier current in human capillary endothelial cells and effects on resting membrane potential. J Physiol 512: 119-128, 1998
  18. Jun JY, Yeum CH, Yoon PJ, Jang IY, Kim SJ, Kim KW. ATP-sensitive $K^+$ current and its modulation by substance P in gastric myocytes isolated from guinea-pig. Eur J Pharmacol358: 77-83, 1998 https://doi.org/10.1016/S0014-2999(98)00577-9
  19. Knot HJ, Zimmermann PA, Nelson MT. External $K^+$ induced dilations of rat coronary and cerebral arteries involve inward rectifier $K^+$ channels. J Physiol 492: 419-430, 1996
  20. Kuryshev YA, Haak L, Childs GV, Ritchie K. Corticotropin releasing hormone inhibits an inwardly rectiJYing potassium current in rat corticotropes. J Physiol 502: 265-279, 1997
  21. Pape HC. Queer current and pacemaker: the hyperpolarizationactivated cation current in neurons. Annu Rev Physiol 58: 299-327, 1996
  22. Quayle JM, Mccaron JG, Brayden JE, Nelson MT. Inward rectifier $K^+$ currents in smooth muscle cells from rat resistance-sized cerebral arteries. Am J Physiol 265: C1363-C1370, 1993
  23. Quayle JM, Dart C, Standen NB. The properties and distribution of inward rectifier potassium currents in pig coronary arterial smooth muscle cells. J Physiol 494: 715-726, 1996
  24. Quayle JM, Nelson MT, Standen NB. ATP-sensitive and inwardly rectifYing potassium channels in smooth muscle. Physio Rev 77: 1165-1232, 1997
  25. Ransom CB, Sontheimer H. Biophysical and pharmacological characterization of inwardly rectifying $K^+$ currents in rat spinal cord astrocytes. J Neurophysiol 73: 1-14, 1995
  26. Sakmann B, Trube G. Voltage-dependent inactivation of inwardrectiJYing single-channel currents in the guinea-pig heart cell membrane. J Physiol 347: 659-683, 1984
  27. Standen NB, Stanfield PR. Potassium depletion and sodium block of potassium currents under hyperpolarization in frog sartorious muscle. J Physiol 294: 497-520, 1979
  28. Tare M, Prestwich SA, Gordienko DV, Parveen S, Carver JE, Robinson C, Bolton TB. Inwardly rectiJYing whole cell potassium current in human blood eosinophils. J Physiol 506: 303-318, 1988
  29. Yamaguchi K, Nakajima Y, Nakajima S, Stanfields PR. Modulation of inwardly rectiJYing channels by substance P in cholinergic neurons from rat brain in culture. J Physiol 426: 499-520, 1990