Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
권호 표지
DOI QR코드

DOI QR Code

Genetic Similarity and Variation in the Cultured and Wild Crucian Carp (Carassius carassius) Estimated with Random Amplified Polymorphic DNA

  • Yoon, Jong-Man (Department of Marine Biomedical Science, College of Ocean Science and Technology, Kunsan National University) ;
  • Park, Hong-Yang (Department of Animal Science, Kon-kuk University)
  • Received : 2001.07.24
  • Accepted : 2001.12.06
  • Published : 2002.04.01
Download PDF
⟨ Previous Next ⟩

Abstract

Random amplified polymorphic DNA (RAPD) analysis based on numerous polymorphic bands have been used to investigate genetic similarity and diversity among and within two cultured and wild populations represented by the species crucian carp (Carassius carassius). From RAPD analysis using five primers, a total of 442 polymorphic bands were obtained in the two populations and 273 were found to be specific to a wild population. 169 polymorphic bands were also produced in wild and cultured population. According to RAPD-based estimates, the average number of polymorphic bands in the wild population was approximately 1.5 times as diverse as that in cultured. The average number of polymorphic bands in each population was found to be different and was higher in the wild than in the cultured population. Comparison of banding patterns in the cultured and wild populations revealed substantial differences supporting a previous assessment that the populations may have been subjected to a long period of geographical isolation from each other. The values in wild population altered from 0.21 to 0.51 as calculated by bandsharing analysis. Also, the average level of bandsharing values was $0.40{\pm}0.05 $ in the wild population, compared to $0.69{\pm}0.08$ in the cultured. With reference to bandsharing values and banding patterns, the wild population was considerably more diverse than the cultured. Knowledge of the genetic diversity of crucian carp could help in formulating more effective strategies for managing this aquacultural fish species and also in evaluating the potential genetic effects induced by hatchery operations.

Keywords

References

  1. Bartish, I. V., L. P. Garkava, K. Rumpunen and H. Nybom. 2000. Phylogenetic relationships and differentiation among and within populations of Chaenomeles Lindl. (Rosaceae) estimated with RAPDs and isozymes. Theor. Appl. Genet. 101:554-561. https://doi.org/10.1007/s001220051515
  2. Bouza, C., L. Sanchez and P. Martinez. 1997. Gene diversity analysis in natural populations and cultured stocks of turbot (Scophthalmus maximus L.). Anim. Genet. 28:28-36. https://doi.org/10.1111/j.1365-2052.1997.00070.x
  3. Cagigas, M. E., E. Vazquez, G. Blanco and J. A. Sánchez. 1999. Combined assessment of genetic variability in populations of brown trout (Salmo trutta L.) based on allozymes, microsatellites and RAPD markers. Mar. Biotechnol. 1:286-296. https://doi.org/10.1007/PL00011778
  4. Coughlan, J. P., A. K. Imsland, P. T. Galvin, R. D. Fitzgerald, G. Naevdal and T. F. Cross. 1998. Microsatellite DNA variation in wild populations and farmed strains of turbot from Ireland and Norway: a preliminary study. J. Fish Biol. 52:916-922. https://doi.org/10.1111/j.1095-8649.1998.tb00592.x
  5. Gjedrem, T. 1992. Breeding plans from rainbow trout. Aqua. 47:245-256.
  6. Hallerman, E. M. and J. S. Beckmann. 1988. DNA-level polymorphism as a tool in fisheries science. Can. J. Fish. Aquat. Sci. 45:1075-1087. https://doi.org/10.1139/f88-131
  7. Huang, B. X., R. Peakall and P. J. Hanna. 2000. Analysis of genetic structure of blacklip abalone (Haliotis rubra) populations using RAPD, minisatellite and microsatellite markers. Mar. Biol. 136:207-216. https://doi.org/10.1007/s002270050678
  8. Hwang, K. C., K. D. Song, T. H. Kim, D. K. Jeong, S. H. Sohn, H. S. Lillehoj and J. Y. Han. 2001. Genetic linkage mapping of RAPD markers segregating in Korean ogol chicken-White leghorn backcross population. Asian-Aust. J. Anim. Sci. 14:302-306. https://doi.org/10.5713/ajas.2001.302
  9. Jeffreys, A. J. and D. B. Morton. 1987. DNA fingerprints of dogs and cats. Anim. Genet. 18:1-15. https://doi.org/10.1111/j.1365-2052.1987.tb00739.x
  10. Li, X., K. Li, B. Fan, Y. Gong, S. Zhao, Z. Peng and B. Liu. 2000. The genetic diversity of seven pig breeds in China, estimated by means of microsatellites. Asian-Aus. J. Anim. Sci. 13:1193-1195. https://doi.org/10.5713/ajas.2000.1193
  11. Lumaret, R., M. Amane, N. Ouazzani, L. Baldoni and C. Debain. 2000. Chloroplast DNA variation in the cultivated and wild olive taxa of the genus Olea L. Theor. Appl. Genet. 101:547-553. https://doi.org/10.1007/s001220051514
  12. Mengistu, L. W., G. W. Mueller-Warrant and R. E. Barker. 2000. Genetic diversity of Poa annua in western Oregon grass seed crops. Appl. Genet. 101:70-79. https://doi.org/10.1007/s001220051451
  13. Mohd-Azmi, M., A. S. Ali and W. K. Kheng. 2000. DNA fingerprinting of red jungle fowl, village chicken and broilers. Asian-Aust. J. Anim. Sci. 13:1040-1043. https://doi.org/10.5713/ajas.2000.1040
  14. Smith, P. J., P. G. Benson and S. M. McVeagh. 1997. A comparison of three genetic methods used for stock discrimination of orange roughy, Hoplostethus atlanticus:allozymes, mitochondrial DNA, and random amplified polymorphic DNA. Fish. Bull. 95:800-811.
  15. Vierling, R. A., Z. Xiang, C. P. Joshi, M. L. Gilbert and H. T. Nguyen. 1994. Genetic diversity among elite Sorghum lines revealed by restriction fragment length polymorphisms and random amplified polymorphic DNAs. Theor. Appl. Genet. 87:816-820. https://doi.org/10.1007/BF00221133

Cited by

  1. Mitochondrial DNA variation in wild populations of Leporinus elongatus from the Paraná River basin vol.26, pp.1, 2003, https://doi.org/10.1590/S1415-47572003000100006
  2. Geographic Variations and Genetic Distance of Three Geographic Cyclina Clam (Cyclina sinensis Gmelin) Populations from the Yellow Sea vol.16, pp.4, 2012, https://doi.org/10.12717/DR.2012.16.4.315
  3. Genetic Variations between Hairtail (Trichiurus lepturus) Populations from Korea and China vol.17, pp.4, 2013, https://doi.org/10.12717/DR.2013.17.4.363
  4. Molecular cloning and expression patterns of two tumor necrosis factor alpha genes in crucian carp (Carassius carassius) vol.49, pp.1, 2015, https://doi.org/10.1134/S0026893315010021
  5. Genetic Diversity in Populations of Silver Crucian Carp Carassius auratus gibelio (Cyprinidae, Cypriniformes), Depending on the Type of Reproduction and Reservoir Size vol.44, pp.10, 2017, https://doi.org/10.1134/S1062359017100181
  6. 붕어(Carassius auratus Linnaeus)와 떡붕어(C. cuvieri Temminck and Schlegel)의 유전적 비교 vol.48, pp.5, 2002, https://doi.org/10.5187/jast.2006.48.5.637
  7. Genetic characterization of Golden mahseer (Tor putitora) populations using mitochondrial DNA markers vol.26, pp.1, 2002, https://doi.org/10.3109/19401736.2013.823170
  8. Genetic Distances of Crucian Carp Populations analyzed by PCR Approach vol.20, pp.2, 2002, https://doi.org/10.12717/dr.2016.20.2.157
  9. Genetic Distances of Scallop (Chlamys farreri) Populations investigated by PCR Procedure vol.21, pp.4, 2017, https://doi.org/10.12717/dr.2017.21.4.435
  10. Genetic Distances in Two Gracilaria Species (Gracilariaceae, Rhodophyta) Identified by PCR Technique vol.22, pp.4, 2002, https://doi.org/10.12717/dr.2018.22.4.393
  11. Genetic Distances between Two Echiuran Populations Discriminated by PCR vol.23, pp.4, 2019, https://doi.org/10.12717/dr.2019.23.4.377
  12. Genetic Distances of Rainbow Trout and Masu Salmon as Determined by PCR-Based Analysis vol.24, pp.3, 2002, https://doi.org/10.12717/dr.2020.24.3.241