Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
권호 표지
DOI QR코드

DOI QR Code

Albumin Fractions from Different Species Stimulate In Vitro Progesterone Production by Granulosa Cells in Buffalo

  • Taneja, R. (Division of Animal Biochemistry, National Dairy Research Institute) ;
  • Bansal, P. (Division of Animal Biochemistry, National Dairy Research Institute) ;
  • Sharma, M.K. (Division of Animal Biochemistry, National Dairy Research Institute) ;
  • Singh, D. (Division of Animal Biochemistry, National Dairy Research Institute)
  • Received : 2002.04.10
  • Accepted : 2002.08.22
  • Published : 2002.11.01
Download PDF
⟨ Previous Next ⟩

Abstract

The ovarian follicular fluid was found to contain steroidogenesis stimulatory protein similar to albumin from human and buffalo. Therefore, the albumins from various species, commercial and purified, were studied for their steroidogenic effect on progesterone secretion by granulosa cells from buffalo ovaries, during culture. A dose of $20{\mu}g$ of bovine serum albumin was optimum to exhibit maximum progesterone secretion on day 6 of culture, in medium ($350{\mu}l$) containing $10^5$ cells. Among commercial albumins, chicken albumin showed highest effect on progesterone secretion, which was followed by albumins from goat, bovine, human, sheep and rat, respectively at day 6 of culture. The albumins were also purified from blood serum of buffalo, goat and rat using salt fractionation, ion-exchange chromatography, gel filtration and SDS-PAGE. The highest stimulatory effect on progesterone secretion was shown by albumin purified from buffalo blood serum and lowest by that from rat blood. Comparatively the buffalo and goat albumins were more biologically active than commercial albumins. The presence of some active molecules conjugated with freshly purified albumins may be responsible for better stimulatory effect.

Keywords

References

  1. Bendel, J .J., D. X. Lobb, A. Chuma, M. Gyster and J. H. Dorrinton. 1988. Bovine theca cells secrete factor(s) that promote granulosa cell proliferation. Biol. Reprod. 38: 780-785. https://doi.org/10.1095/biolreprod38.4.790
  2. Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the protein dye binding. Anal. Biochem. 27: 248-254. https://doi.org/10.1016/0003-2697(76)90527-3
  3. Darcel, C. L. E. Q. 1987. Minireview on the heterogeneity of serum albumin. Biochemistry 19(4):295-301.
  4. Fortune, J. E. 1994. Ovarian follicular growth and development in mammals. Biol. Reprd. 50:225-232. https://doi.org/10.1095/biolreprod50.2.225
  5. Hsueh, A. J. W. and G. F. Erickson. 1979. Extra pituitary action of gonadotropin releasing hormone: Direct inhibition of ovarian steroidogenesis. Science 204:854-855. https://doi.org/10.1126/science.375393
  6. Kamboj, M. and B. S. Prakash. 1993. Relationship of progesterone in plasma and whole milk of buffaloes during cyclicity and early pregnancy. Trop. Anim. Health Prod. 25:185-192. https://doi.org/10.1007/BF02236239
  7. Khan, S. A., P. Hallin, J. Bartlett, Ch. De Geyer and E. Nieschlag. 1988. Characterization of factor from human follicular fluid, which stimulate Leydig cell testosterone production. Acta Endocrinol. 118:283-293.
  8. Khan, S. A., C. Keck, T. Gludesmann and E. Nieschlag. 1990. Isolation of a protein from human ovarian follicular fluid which exerts major stimulatory effects on in vitro steroid production of testicular, ovarian and adrenal cells. Endocrinology 126(6):3043-3052. https://doi.org/10.1210/endo-126-6-3043
  9. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of head of bacteriophage $T_4$. Nature 227:680-685. https://doi.org/10.1038/227680a0
  10. Melsert, R., J. W. Hoogerbrugge and F. F. G. Rommerts. 1988. The albumin fraction of rat testicular fluid stimulates steroid production by isolated Leydig cells. Mol. Cell. Endocrinol. 59:221-231. https://doi.org/10.1016/0303-7207(88)90107-4
  11. Nayudu, P. L. and S. M. Osborn. 1992. Factors influencing the rate of preantral and antral growth of mouse ovarian follicles in vitro. J. Reprod. Fertil. 95:349-362. https://doi.org/10.1530/jrf.0.0950349
  12. Peters, T. Jr. 1985. Serum albumin. Adv. Protein Chem. 37:161-245. https://doi.org/10.1016/S0065-3233(08)60065-0
  13. Shalgi, R., P. F. Kracier, A. Rimon, M. Pinto and N. Soferman. 1973. Proteins of human follicular fluid: The blood follicle barrier. Fert. Steril. 24:429-434.
  14. Sluss, P. M., P. W. Fletcher and L. E. Jr. Reichert. 1983. Inhibition of 125I-hFSH binding to receptor by a low MW fraction of bovine follicular fluid: Inhibitor concentration is related to biochemical parameters of follicular development. Biol. Reprod. 29:1105-1113. https://doi.org/10.1095/biolreprod29.5.1105
  15. Snedecor, G. W. and W. G. Cochran. 1967. Statistical Methods. Oxford and IBH Publishing company, New Delhi, India.
  16. Stroker, R., A. F. McDonagh, A. N. Glazer and B. N. Amer. 1990. Antioxidant activities of bile pigments: biliverdin and bilirubin. Methods Enzymol. 186:301-309. https://doi.org/10.1016/0076-6879(90)86123-D
  17. Vinze, M. 1998. Studies on follicular fluid proteins and their effect on in vitro steroidogenesis in buffaloes. Ph.D. Thesis, National Dairy Research Institute (Deemed University), Karnal, Haryana.
  18. Wickings, E. J. 1986. Gonadotrophic control of steroidogenesis in human-granulosa lutein cells. J. Reprod. Fertil. 76:677-684. https://doi.org/10.1530/jrf.0.0760677
  19. Yuji, O., M. Nobuyuki, W. Akihiko, N. Yuji and S. Yusuke. 1993. Role of free radical scavenger system in aromatase activity of human ovary. Horm. Res. 39: (suppl.1):22-27. https://doi.org/10.1159/000182753