Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Effects of three kinds of Radix Rehmanniae Water Extract in Cultured Rat Myocardial Cells

삼종 지황 추출물이 배양 심근세포에 미치는 영향

  • Hwang In Jin (Department of Physiology, College of Oriental Medicine, Wonkwang University) ;
  • Kwon Kang Beom (Department of Physiology, College of Oriental Medicine, Wonkwang University) ;
  • Cho Hyun Ik (Department of Physiology, College of Oriental Medicine, Wonkwang University) ;
  • Min Young Gi (Department of Physiology, College of Oriental Medicine, Wonkwang University) ;
  • Heo Jae Hyuk (Department of Physiology, College of Oriental Medicine, Wonkwang University) ;
  • Kim Gu Hwan (Department of Physiology, College of Oriental Medicine, Wonkwang University) ;
  • Ryu Do Gon (Department of Physiology, College of Oriental Medicine, Wonkwang University)
  • 황인진 (원광대학교 한의과대학 생리학교실) ;
  • 권강범 (원광대학교 한의과대학 생리학교실) ;
  • 조현익 (원광대학교 한의과대학 생리학교실) ;
  • 민영기 (원광대학교 한의과대학 생리학교실) ;
  • 허재혁 (원광대학교 한의과대학 생리학교실) ;
  • 김구환 (원광대학교 한의과대학 생리학교실) ;
  • 류도곤 (원광대학교 한의과대학 생리학교실)
  • Published : 2002.12.01
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Abstract

To test the protective effect of herbal medicine on myocardial damage against oxygen free radical-induced myocardiotoxicity, cytotoxicity was examined using MTT, Beating rate and DNA synthesis assay in the presence of water extract of three kinds of Radix Rehmanniae. Myocardial toxicity was evaluated in neonatal rat myocardiocytes in cultures. The results of these experiments were obtained as follows : Xanthine oxydase/hypoxanthine resulted in a decrease in viability, beating rate and DNA synthesis in cultured myocardial cells. Radix Rehmanniae Recens(生地黃, RRR) water extract shows effects of protection from the cardiocyte toxicity induced by xanthine oxydase/hypoxanthine treatment such as increases in beating rate. Radix Rehmanniae Preparat(熟地黃, RRP) water extract shows effects of protection from the cardiocyte toxicity induced by xanthine oxydase/hypoxanthine treatment such as increases in DNA synthesis. These results show that xanthine oxydase/hypoxanthine elicits toxic effects in cultured myocardial cells derived from neonatal rat, and suggest that water extract of three kinds of Radix Rehmanniae is very effective in the prevention of xanthine oxydase/hypoxanthine-induced cardiotoxicity.

Keywords

References

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