Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Screening of Antimicrobial Activity from Differential Extracts of Allii sativi Bulbus

대산의 분획별 추출물에서 항균활성 검색

  • Kim Hee Seok (Professional Graduate School of Oriental Medicine, Medial School Wonkwang University) ;
  • Bae Heung Mo (Professional Graduate School of Oriental Medicine, Medial School Wonkwang University) ;
  • Kim Shin Moo (Department of Clinical Pathology, Wankwang Health Science College) ;
  • Lee Hyun Ok (Department of Dental Hygiene Wankwang Health Science College) ;
  • Kim Ki young (Professional Graduate School of Oriental Medicine, Department of Pathology, Medial School Wonkwang University)
  • 김희석 (원광대학교 한의학전문대학원) ;
  • 배흥모 (원광대학교 한의학전문대학원) ;
  • 김신무 (원광보건대학 임상병리과) ;
  • 이현옥 (원광보건대학 치위생과) ;
  • 김기영 (원광대학교 한의학전문대학원, 원광대학교 의과대학 병리학교실)
  • Published : 2002.12.01
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Abstract

Allii sativi Bulbus(garlic) have been shown to possess medicinal value, in particular, antimicrobial activity. In this study, we compared the efficacy on some pathogenic bacteria and fungus among several different extracts(water, hexane, ethyl acetate, methanol, chloroform) of Allii sativi Bulbus. Animal pathogenic bacteria and fungus(S. gallinarium: KCTC 2441, S. flexneri: KCTC 2361, E. cloacae: KCTC 2006, K. pneumonia: KCTC 2208, C. albicans: KCTC 1940) were used to test by measurement of minimum inhibitory concentrations(MIC) and disc diffusion. Allii sativi Bulbus were cut and mixed with water at 37℃ about 24 h and filtered, and several different solvents(hexane, chloroform, ethyl acetate, methanol) were respectively added to separate the fraction of each solvent. The antimicrobial(bacteriocidal) and antifungal effect were apparently shown from water extract, hexane and ethyl acetate extract against using strains(Staphylococcus gallinarium, Shigella flexneri, Enterobacter doacae, Klebsiella pneumonia, Candida albicans). Especially, the water extract showed the superior efficacy. And the clear zone size of water extract (11～27 mm) was greater than that of gentamycin, hexane extract and ethyl acetate extract against S. gallinarium. S. flexneri, K. pneumonia and C. albicans. Minimum inhibitory concentrations(MIC) of water extract appeared to around 2.0～7.5 ㎎/㎖ against S. gallinarium, S. flexneri, E. cloacae and K. pneumonia. The greater activity was shown by water extract because the MIC of water extract for C. albicans observed in very low concentration(<1.0 ㎎/㎖) compared to hexane(5.0 ㎎/㎖) and ethyl acetate(10.0 ㎎/㎖). Therefore, these results exhibited that water extract of Allii sativi Bulbus have stronger antimicrobial activity than hexane and ethyl acetate extract, and may be useful as topical medicine of superficial infections causing C. albicans as well as antifungal agents.

Keywords

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