Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Cardiotoxicity and Effect of Benincasae Semen on the Reactive Oxygen Species

활성산소에 의한 심근독성 및 동과의 보호효과에 관한 연구

  • Published : 2002.12.01
Download PDF
⟨ Previous Next ⟩

Abstract

To examine the cardiotoxicity of glucose oxidase(GO) in cultured myocardial cells, cardiotoxicity was measured by MTT assay. Myocardial cells were treated with 1～50 mU/ml GO for 5 hours. The cardioprotective effect of Benincasae Semen(BS) was measured by MTT assay in these cultrures. Cell viability was significantly decreased in dose- and time-dependently after myocardial cells were exposed to 30mU/ml GO for 5 hours. In the cardioprotective effect of BS on the cardiotoxicity induced by GO, BS prevented the cardiotoxicity induced by GO in these cultures. From these results, it suggests that GO had cytotoxic effect in cultured myocardial cells and herb extract, BS showed protective effect on GO-induced cardiotoxicity.

Keywords

References

  1. FASEB J v.8 Oxidative DNA damage correlates with oxygen consumption in humans. Loft S;Astrup A;Buemann B;Poulsen HE
  2. Ann Neurol v.28 Mutation in Cu/Zn superoxide dismutase gene are associated acid metabolism in amyotrophic lateral sclerosis. Rosen D;Siddique T;Patterson D;Figlewiez D;Sapp P;Hentati A; Donaldson D;Goto J;O Regan J;deng H;Rahmani Z;Krrizus A(et al.)
  3. Circ Res v.65 Alterations in cardiac sarcoplasmic reticulum calcium transport in the post-ischemic "stunned myocardium. Krause SM;Jacobus WE;Becker LC
  4. Toxic in Vitro v.9 Cardiotoxicity of dichromomethane in rats and in cultured rat cardiac myocyte. Hoffman P;Muller SP;Heinroth K;Buchner E;Richards R;Toraason M
  5. J Neurochem v.51 Excitatory amino acid release from rat hippocampal slices as a consequence of free radical formation. Pellegrini-Giampietro DE;Cherici G;Alesiani M;Carla V;Moroni F
  6. J M Cell Cardiol v.19 Conjugated dienes in ischemic and reperfused myocardium free radical mediated injury. Romaschin AD;Rebeyka I;Wilson GJ;Mickle DAG
  7. FASEB J v.9 Oxidative processes and antioxidative defense mechanism in the aging brain Reiter RJ
  8. Toxicologist v.11 Intracellular calcium transients in cardiac myocytes exposed to carbon tetrachloride (Abstract). Toraason M.;Breitenstein M.
  9. J Neurosci Res v.37 Oxygen radical-induced neurotoxicity in spinal cord neuron cultures Michikawa M;Lim KT;McLarnon JG;Kim SU
  10. FASEB J v.9 Oxidative processes and antioxidative defense mechanism in the aging brain Reiter RJ
  11. Toxic In Vitro v.2 Coparisons of two in vitro cytotoxzicity assays-The neutral red(NR) asnd tetrazolium MTT test. Borenfreund E;Babich H;Martin-Alcuacil N
  12. Cir Res v.58 Superoxide dimutase plus catalase improve contractile function in the canine model of "stunned myocardium". Przyklenk K.;Kloner R. A.
  13. Wonkwang J Environ v.4 Effects of methylmercury on the fetal mouse cerebral neurons. Park ST;Kim JJ;Mun YJ;Lim KT;Choi MK;Chung YT
  14. 경희대학교 논문집 v.14 단삼보혈탕 및 보화환이 위궤양에 미치는 영향 박동원
  15. 생약학회지 v.31 오배자 항산화 활성성분과 자유라디칼 소거효과. 차배천;이승배;임태진;이광희