Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Effect of Silsosangami on Platelet Aggregation, Hemolysis and Inducible Nitric Oxide Synthase

  • Kim Chang Hwan (Department of Diagnostics, College of Oriental Medicine, Dongguk University) ;
  • Kim Han Geu (Department of Diagnostics, College of Oriental Medicine, Dongguk University) ;
  • Ahan Jong Chan (Department of Diagnostics, College of Oriental Medicine, Dongguk University) ;
  • Lee Soo Kyung (Department of Diagnostics, College of Oriental Medicine, Dongguk University) ;
  • Chung Tae Wook (Department of Diagnostics, College of Oriental Medicine, Dongguk University) ;
  • Kim June Ki (Department of Pathology, College of Oriental Medicine, Dongguk University) ;
  • Choi Dall Yeong (Department of Pathology, College of Oriental Medicine, Dongguk University) ;
  • Kim Cherl Ho (Department of Biochemistry, College of Oriental Medicine, Dongguk University) ;
  • Park Won Hwan (Department of Diagnostics, College of Oriental Medicine, Dongguk University)
  • Published : 2002.12.01
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Abstract

Silsosangami(SSG) is a formula of treaditional korean medicines as an effective biological response modifier for augmenting host homeostasis of body circulation. Little is known of the biological activity of SSG and previous studies have focused mainly on their anti-thrombosis8). There is a growing interest in the pharmacological potential of the SSG due to the recent finding by our group that SSG and each constituent herbs of SSG were able to inhibit NO and prostaglandin E2 (PGE2) synthesis in murine peritoneal macrophages stimulated with bacterial endotoxin. In this paper, the effects of SSG on platelet aggregation and hemolysis in human blood were studied. SSG provoked remarkable inhibiting effect on platelet aggregation, and APTT were sensitive to the presence of this SSG. Using an in vitro system, APTT was delayed with the increment of the concentrations of these seven compounds. These results suggested that SSG might be used as a novel antithrombotic therapeutic agents in post-myocardial infarction. A SSG reduced NO production in mouse peritoneal macrophages stimulated with lipopolysaccharide, without the influence on the activity of iNOS being observed. SSG significantly reduced mouse paw edema induced by carrageenan. Western blot analysis showed that SSG reduced the expression of iNOS. The results indicate that SSG exerts anti-inflammatory effects related to the inhibition of NO production, which could be due to a decreased expression of iNOS.

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