Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Effects of Rhizoma Coptidis Water Extract in Cultured Rat Myocardial Cells

황연 추출물이 산소자유기에 의해 손상된 배양 심근세포에 미치는 영향

  • Yang Sang Cheol (Department of Physiology, College of Oriental Medicine, Wonkwang University) ;
  • Kwon Kang Beam (Department of Physiology, College of Oriental Medicine, Wonkwang University) ;
  • Cho Hyun Ik (Department of Physiology, College of Oriental Medicine, Wonkwang University) ;
  • Min Young Gi (Department of Physiology, College of Oriental Medicine, Wonkwang University) ;
  • Heo Jae Hyuk (Department of Physiology, College of Oriental Medicine, Wonkwang University) ;
  • Kim Gu Hwan (Department of Physiology, College of Oriental Medicine, Wonkwang University) ;
  • Ryu Do Gon (Department of Physiology, College of Oriental Medicine, Wonkwang University)
  • 양상철 (원광대학교 한의과대학 생리학교실) ;
  • 권강범 (원광대학교 한의과대학 생리학교실) ;
  • 조현익 (원광대학교 한의과대학 생리학교실) ;
  • 민영기 (원광대학교 한의과대학 생리학교실) ;
  • 허재혁 (원광대학교 한의과대학 생리학교실) ;
  • 김구환 (원광대학교 한의과대학 생리학교실) ;
  • 류도곤 (원광대학교 한의과대학 생리학교실)
  • Published : 2002.10.01
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Abstract

To test the protective effect of herbal medicine on myocardial damage against oxygen free radical-induced myocardiotoxicity, cytotoxicity was examined using MTT, Beating rate and TSARS assay in the presence of water extract of Rhizoma Coptidis. Myocardial toxicity was evaluated in neonatal rat myocardiocytes in cultures. The results of these experiments were obtained as follows: Xanthine oxydase/hypoxanthine resulted in a decrease in viability, beating rate and in a increase in lipid peroxidation in Cultured myocardial cells. Rhizoma Coptidis water extract shows effects of protection from the cardiocyte toxicity induced by xanthine oxydase/hypoxanthine treatment such as increases in beating rate. Rhizoma Coptidis water extract shows effects of protection from the cardiocyte toxicity induced by xanthine oxydase/hypoxanthine treatment such as decreases in lipid peroxidation. These results show that xanthine oxydase/hypoxanthine elicits toxic effects. in cultured myocardial cells derived from neonatal rat, and suggest that water extract of Rhizoma Coptidis is very effective in the prevention of xanthine oxydase/hypoxanthine-induced cardiotoxicity.

Keywords

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