Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Effect of Ramulus et uncus uncariae on Glucose Oxidase-Induced Toxicity in Cultured Cerebral Neurons

조구등이 Glucose Oxidase로 손상된 대뇌신경세포에 미치는 효과

  • Kim Hyeong Soo (School of Medicine, Wonkwang University) ;
  • Lee Yong Suk (School of Medicine, Wonkwang University) ;
  • Oh Suk Kyu (School of Medicine, Wonkwang University) ;
  • Lee Kang Chang (Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Lee Geon Mok (Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Lee Jeong (Wonkwang health Science College) ;
  • Lee Sang Bork (Wonkwang health Science College) ;
  • Kim Jong Ho (Wonkwang health Science College) ;
  • Yu Jun Ki (College of Oriental Medicine. Wonkwang University) ;
  • Kang Young Seong (College of Oriental Medicine. Wonkwang University) ;
  • Kim Sung Soo (College of Oriental Medicine. Wonkwang University) ;
  • Song Ho Jun (College of Oriental Medicine. Wonkwang University) ;
  • Park Seung Taeck (School of Medicine, Wonkwang University)
  • Published : 2002.10.01
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Abstract

To examine the cytotoxic effect of glucose oxidase(GO) in cultured mouse cerebral neurons, cytotoxicity was measured by MTT assay after cultured nerve cells were incubated for 3 hours in the media containing 1 ～ 60mU/ml concentrations of GO. In addition, the neuroprotective effect of Ramulus et uncus uncariae(REUU) was determined by MTT assay in these cultrures. Cell viability was remarkably decreased in a dose- and time-dependent manner after cultured mouse cerebral neurons were exposed to 30mU/ml GO for 3 hours. In the neuroprotective effect of REUU on GO-induced toxicity, REUU blocked the GO-mediated neurotoxicity in these cultures. From above the results, it suggests that GO is toxic in cultured mouse cerebral neurons and selective herb extract such as REUU is effective in prevetion of the neurotoxicity induced by GO.

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