동의생리병리학회지 (Journal of Physiology & Pathology in Korean Medicine)

  • 제16권5호
  • /
  • Pages.1025-1034
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  • 2002
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  • 1738-7698(pISSN)
  • /
  • 2288-2529(eISSN)
대한동의생리학회·한의병리학회 ( The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine)
권호 표지

육미지황탕가미방이 흰쥐의 기억능력과 중추신경계 유전자 발현에 미치는 영향

Effect on Gene Expression Profile of Rat Hippocampus Caused by Administration of Memory Enhancing Herbal Extract

  • 최보업 (경희대학교 한의과대학 생리학교실) ;
  • 배현수 (경희대학교 한의과대학 생리학교실) ;
  • 신민규 (경희대학교 한의과대학 생리학교실) ;
  • 홍무창 (경희대학교 한의과대학 생리학교실)
  • Choi So Eop (Department of Physiology, College of Oriental Medicine, Kyung Hee University) ;
  • Bae Hyun Su (Department of Physiology, College of Oriental Medicine, Kyung Hee University) ;
  • Shin Min Kyu (Department of Physiology, College of Oriental Medicine, Kyung Hee University) ;
  • Hong Moo Chang (Department of Physiology, College of Oriental Medicine, Kyung Hee University)
  • 발행 : 2002.10.01
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초록

The herbal extract (YMT_02) is a modified herbal extracts from Yukmijihwang-tang (YMJ) to promote memory-enhancing. The YMJ extracts has been widely used as an anti-aging herbal medicine for hundred years in Asian countries. The purpose of this study is to; 1) quantitatively evaluate the memory-enhancing effect of YMT_02 by behavior task, 2) identify candidate genes responsible for enhancing memory by cDNA microarray and 3) assess the anti-oxidant effect of YMT_02 on PC12 cell. Memory retention abilities are addressed by passive avoidance task with Sprague-Dawley (SD) male rat. Before the training session, the rats are subdivided into four groups and administrated with YMT_02, Ginkgo biloba, Soya lecithin and normal saline for 10 days. The retention test was performed. 24 hours after the training session. The retention time of the YMT_02 group was significantly (p<0.05) delayed (～100%), whereas Ginkgo biloba and Soya lecithin treatment delayed 20% and 10% respectively. The hippocampi of YMT_02 and control group were dissected and mANA was further purified. After synthesizing cDNA using oligo-dT primer, the cDNA were applied to Incyte rat GEMTM 2 cDNA microarray. The microarray results show that prealbumin(transthyretin), phosphotidylethanolamine N-methyltransferase, and PEP-19 are expressed abundantly in the YMT_02 treated group. Especially, PEP-19 is a neuron-specific protein, which inhibits apoptotic processes in neuronal cell. On the other hand, transcripts of RAB15, glutamate receptor subunit 2 and CDK108 are abundant in control group. Besides, neuronal genes involved in neuronal death or neurodegeneration such as neuronal-pentraxin and spectrin are abundantly expressed in control group. Additionally, the YMT_02 shows an anti oxidative effect in the PC12 cell. The list of differentially expressed genes may implicate further insight on the action and mechanism behind the memory-enhancing effect of herbal extracts YMT_02, for example, anti-apoptotic, anti-oxidative, and neuroprotective effects.

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