Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Effects of Cordyceps militaris on $CCl_4$ - Induced Liver Damage and Cancer Cell (HepG2 Cell) Growth

동충하초가 사염화탄소로 유발된 간 손상 및 간암세포증식에 미치는 영향

  • Kim San (Department of Opthamology Otoiaryngology and Dermatology, Oriental Medical Collage, Wonkwang University) ;
  • Hwang Choong yeon (Department of Opthamology Otoiaryngology and Dermatology, Oriental Medical Collage, Wonkwang University) ;
  • Kim Nam kwen (Department of Opthamology Otoiaryngology and Dermatology, Oriental Medical Collage, Wonkwang University) ;
  • Park Min cheul (Department of Opthamology Otoiaryngology and Dermatology, Oriental Medical Collage, Wonkwang University) ;
  • Kim Jin (Department of Opthamology Otoiaryngology and Dermatology, Oriental Medical Collage, Wonkwang University)
  • 김산 (원광대학교 한의과대학 외관과교실) ;
  • 황충연 (원광대학교 한의과대학 외관과교실) ;
  • 김남권 (원광대학교 한의과대학 외관과교실) ;
  • 박민철 (원광대학교 한의과대학 외관과교실) ;
  • 김진 (원광대학교 한의과대학 외관과교실)
  • Published : 2002.08.01
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Abstract

Cordyceps militaris has been known as a Chinese traditional medicine for the treatment of tuberculosis, asthma, kidney disease, debility and fatigue etc. This study was attempted to investigate the therapeutic effect of C. militaris extract on the cytotoxic activity of HepG2, human hepatocellular carcinoma cells and the liver damage induced by carbon tetrachloride in SD rats. C. militaris extracts inhibited significantly the proliferation of HepG2 cells in vitro. Carbon tetrachloride(CCl₄) caused a significant an increase in liver weight, serum aspartate aminotransferase(AST) and alanine aminotransferase(ALT) activity, alkaline phosphatase(ALP), serum thiobarbituric acid reactive substances (TBARS), microsomal TBARS, and decrease in microsomal detoxification enzymes (cytochrome P-450, P-450 reductase, cytochrome b5, b5 reductase). TBARS and ALP in serum pretreated with C. militaris extracts (300mg/kg/day, 600mg/kg/day) was significantly reduced compared to control group(CCl₄). Cytochrome b5 and b5 reductase activities were significantly increased in CM300 (300 mg/kg/day) and CM600 group(600 mg/kg/day), and cytochrome P-450 reductase was significantly increased in CM300 group. Pretreatment (100, 300, and 600 mg/kg/day for 7 days) of C. militaris with CCl₄ was significantly inhibited the accumulation microsomal TBARS and the significantly increased in the cytochrome P-450 activity. These results suggested that C. militaris (300mg/kg/day for 7 days) has appreciable therapeutic effect on CCl₄ induced hepatotoxicity.

Keywords

References

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