Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Effect of Radix Polygoni Multiflori on Cultured Vascular Endotherial Cells Damaged by Xanthine Oxidase and Hypoxanthine

하수오가 Xanthine Oxidase와 Hypoxanthine에 의해 손상된 혈관내피세포에 미치는 영향에 관한 연구

  • Published : 2002.08.01
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Abstract

To clarify the vasculotoxicity of reactive oxygen intermediate(ROI) in cultured vascular endotherial cells(VEC), of mouse, cytototoxicity was measured by MTS assay after VEC was incubated to 10～80mU/ml xanthine oxidase(XO) and hypoxanthine(HX) for 2 hours. and also, the protective effect of Radix Polygoni Multiflori(RPM) was determined by MTT assay in these cultrures. Cell viability was positively decreased dose-, and time-dependently, after the treatment with 40mU/ml XO/0.1 mM HX to cultured VEC for 2 hours. In the vasculoprotective effect of RPM on the toxicity induced by XO/HX, RPM prevented the XO/HX-induced cytotoxicity in these cultures. From above the results, it suggests that XO/HX is toxic in cultured VEC and herb extract, RPM has protective effect against the vasculotoxicity induced by XO/HX.

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