Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Effect of 1,2,3,4,6-penta-O-galloyl-$\beta$-D-glucose isolated from Galla Rhois on gap junctional intercellular communication and antiangiogenic activity

오배자 BuOH층에서 분리한 1,2,3,4,6-penta-O-galloyl-$\beta$-D-glucose의 세포간교통 회복 및 혁관형성 억제작용에 대한 효과

  • Huh Jeong Eun (Department of Oncology, Graduate School of East-West Medial Science, KyungHee University) ;
  • Lee Hyo Jung (Department of Oncology, Graduate School of East-West Medial Science, KyungHee University) ;
  • Song Gyu Yong (Department of Oncology, Graduate School of East-West Medial Science, KyungHee University) ;
  • Cha Bae Cheon (Division of Applied Animal Science, Sangjj University) ;
  • Kim Han Sung (College of Oriental Medicine, Daejon University) ;
  • Yoo Dong Youl (College of Oriental Medicine, Daejon University) ;
  • Ryu Shi Yong (Korea Research Institute of Chemical Technology) ;
  • Kim Sung Hoon (Department of Oncology, Graduate School of East-West Medial Science, KyungHee University)
  • 허정은 (경희대학교 동서의학대학원) ;
  • 이효정 (경희대학교 동서의학대학원) ;
  • 송규용 (경희대학교 동서의학대학원) ;
  • 차배천 (상지대학교 생명과학대학 응용동물과학부) ;
  • 김한성 (대전대학교 한의과대학) ;
  • 유동열 (대전대학교 한의과대학) ;
  • 유시용 (대전대학교 화학연구소) ;
  • 김성훈 (경희대학교 동서의학대학원)
  • Published : 2002.06.01
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Abstract

Galla Rhois is a gallnut of Rhus javanica Linne used for treatment of diarrhea, hemorrhage, cough, leukorrhea and toxic tumor etc in oriental medicine. For the evaluation of antitumor effect of Galla Rhois, activity based fractionation was done. We isolated an effective compound and identified 1,2,3,4,6-penta-O-galloyl-β-D-glucose(PGG) by photometric analysis such as NMR and MASS. Then, we studied the angiogenic activity of PGG. It showed a cytotoxicity against SK-OV-3, SK-OV-3, HT1080 with IC/sub 50/ of 50 ug/ml approximately. It also effectively inhibited proliferation of HUVEC cells treated by bFGF to 30% of control at 20 ug/ml and cell migration to 80% at 10 ug in a dose dependent fashion. Tube formation of HUVEC cells on matrigel was effectively suppressed from 2.5 ug/ml of concentration by PGG. Moreover, it effectively recovered the dysfunction of gap junctional intercellular communication in WB-F344 rat liver epithelial cells caused by hydrogen peroxide at 4 ug/ml suggesting it potently can inhibit tumor promotion. Taken together, it indicates 1,2,3,4,6-penta-O-galloyl- β -D-glucose has antiangiogenic activity.

Keywords

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