Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Effect of Rhizoma Gastrodiae on Cultured Spinal Motor Neurons Damaged by Oxygen Radicals

천마가 산소자유기로 손상된 생쥐의 배양 척수 운동신경세포에 미치는 영향

  • Son IL Hong (School of Medicine, Wonkwang University) ;
  • Lee Jung Hun (School of Medicine, Wonkwang University) ;
  • kim Sang Su (Institution of Wonkwang Science, Wonkwang University) ;
  • Lee Kang Chang (Department of Graduate School of Oriental Medicine, Wonkwang University) ;
  • Lee Young Mi (School of Medicine, Wonkwang University) ;
  • Hong Gi Youn (School of Medicine, Wonkwang University) ;
  • Moon Hyung Bae (School of Medicine, Wonkwang University) ;
  • Seo Eun A (Medicinal Resources Research Center, Wonkwang University) ;
  • Han Du Seok (College of Dental Medicine, Wonkwang University) ;
  • Shin Min Kyo (College of Oriental Medicine, Wonkwang University) ;
  • Song Ho Joan (College of Oriental Medicine, Wonkwang University) ;
  • Park Seung Taeck (Institution of Wonkwang Science, Wonkwang University)
  • 손일홍 (원광대학교 의과대학) ;
  • 이정헌 (원광대학교 의과대학) ;
  • 김상수 (원광대학교 원광의학연구소) ;
  • 이강창 (원광대학교 한의학전문대학원) ;
  • 이영미 (원광대학교 의과대학) ;
  • 홍기연 (원광대학교 의과대학) ;
  • 문형배 (원광대학교 의과대학) ;
  • 서은아 (원광대학교 의학자원연구센타) ;
  • 한두석 (원광대학교 치과대학) ;
  • 신민교 (원광대학교 한의과대학) ;
  • 송호준 (원광대학교 한의과대학) ;
  • 박승택 (원광대학교 원광의학연구소)
  • Published : 2002.04.01
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Abstract

In order to elucidate the mechanism of cytotoxic effect of oxygen radicals on cultured mouse spinal motor neurons, the neurotoxicity induced by hydrogen peroxide(H₂O₂) was evaluated by MTT assay. The neuroprotective effect of Rhizoma Gastrodiae(RG) against H₂O₂-mediated neurotoxicity was also examined in these cultures by SRB assay. The results were as follows : The value of lethal concentration 50(LC50) of H₂O₂ was estimated at a concentration of 30 uM in these cultures. Cell viability of cultured mouse spinal motor neurons was remarkably decreased by H₂O₂-induced neurotoxicity in a dose- and time-dependent manner. RG was remarkably effective in blocking the neurotoxicity induced by H₂O₂ at a concentration of 120 μM as determined by SRB assay. From above the results, it is suggested that H₂O₂ induce neurotoxicity, and the selective herbal extracted RG was very effective in blocking H₂O₂-mediated neurotoxicity on cultured mouse spinal motor neurons.

Keywords

References

  1. Exp Brain Res v.84 Medial vestibular nucleus in the guinea pig. Ⅱ. Ionic basis of the intrinsic membrane properties in brainstem slices. Serafin M;de waele C;Khateb A;Vidal P;Muhlethaler M https://doi.org/10.1007/BF00231465
  2. J Pharmacol Exp Ther Calcium antagonist effects on low-threshold(T-type) calcium current in rat isolated hippocampal CAI pyramidal neurons. Takahashi K;Akaike N
  3. Exp Brain Res v.50 Short-and long-term modification of vestibulo-ocular response dynamics following unilateral vestibular nerve lesion in the cat. Maioli C;Precht W;Ried S
  4. J Exp Neurol v.124 Mechanisms of neurotrophic factor protection against calcium-and free radical mediated excitotoxic injury :Implications for treating neurogenerative disorders. Mattson MP;Cheng B;Sminth-Swintosky VL https://doi.org/10.1006/exnr.1993.1178
  5. Brain Res v.444 Neuronal activity in the ipsilateral medial vestibular nucleus of the guniea pig following unilateral labyrinthectomy. Smith PF;Curthoys IS https://doi.org/10.1016/0006-8993(88)90939-0
  6. J Neurosci v.10 Glutamate neurotoxicity in vitro : Antagonist pharmacology and intracellular calcium concentrations. Michaels RL;Rothman SM
  7. Brain Res v.521 Characterization and mechanism of glutamate neurotoxicity in primary striatal culture. Freese A;DiFiglia M;Koroshetz WJ;Beal MF;Martin JB
  8. Pharmacol Res v.33 Vestibular compeusation in aged rats with unilateral labyrinthectomy treated with dopaminergic drugs. Drago F;Nardo L;Rampello L;Raffaele R https://doi.org/10.1006/phrs.1996.0020
  9. Neurotoxicol v.17 Methylmercury- in-duced neurotoxicity in cerebral neuron culture is blocked by antioxidants and NMDA receptor antagonists. Park ST;Lim KT;Chung YT;Kim SU
  10. Neuroreport v.3 Pre-treatmnet with a Ca2+ channel antagonist facilitates vestibular compensation. Darlington CL;Smith PF https://doi.org/10.1097/00001756-199202000-00005
  11. Neurosci Lett v.222 Role of vestibulo-cerebellar N-methyl-D-aspartate receptors for behavioral recovery following unilateral labyrinthectomy in rats. Kim MS;Jin BK;Chun SW;Lee MY;Lee SH;Kim JH;Park BR https://doi.org/10.1016/S0304-3940(97)13371-7
  12. J Neurosci v.10 Excitatory amino acid and free radical formation may cooperate in the genesis of ischemia-in- duced neuronal damage. Pellegrini-Giampietro DE;Cherici G;Alesiani M;Carla V;Morroni F
  13. Mol Neurobiol v.5 Excitatory amino acid receptors in normal and abnormal vestibular function. Smith PF;de Waele C;Vidal PP;Darlington CL https://doi.org/10.1007/BF02935559
  14. J Immun Methods v.65 Rapid colorimetric assay for the cellular growth and survival : Application to proliferation and cytotoxic assays. Mosmann T https://doi.org/10.1016/0022-1759(83)90303-4
  15. J Neurosci Res v.37 Oxygen radical-induced neurotoxicity in spinal cord neuron cultures. Michikawa M;Lim KT;McLarnon JG;Kim SU https://doi.org/10.1002/jnr.490370109
  16. Neuropathol Appl Neurobiol v.20 Excitatory amino acids, free radicals and the pathogenesis of motor neurons disease. Zeman S;Lioyd C;Meldrum B;Leigh PN https://doi.org/10.1111/j.1365-2990.1994.tb00963.x