동의생리병리학회지 (Journal of Physiology & Pathology in Korean Medicine)

  • 제16권2호
  • /
  • Pages.272-278
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  • 2002
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  • 1738-7698(pISSN)
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  • 2288-2529(eISSN)
대한동의생리학회·한의병리학회 ( The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine)
권호 표지

관절염 모델에서 백굴채전탕액이 척수와 척수신경절의 Calcitonin Gene-Related Peptide와 Substance P 면역반응에 미치는 영향

The Effects of Herba Chelidonii Extracts on Calcitonin Gene-Related Peptide and Substance P Immunoreactive Response in Spinal Cord and Ganglia of Adjuvant-Induced Arthritis

  • 박종주 (우석대학교 한의과대학 침구학교실) ;
  • 육태한 (우석대학교 한의과대학 침구학교실) ;
  • 송범용 (우석대학교 한의과대학 침구학교실) ;
  • 이광규 (우석대학교 한의과대학 병리학교실) ;
  • 유윤조 (우석대학교 한의과대학 생리학교실) ;
  • 이창현 (우석대학교 한의과대학 해부학교실)
  • Park Jong Joo (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Woosuk University) ;
  • Yook Tae Han (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Woosuk University) ;
  • Song Beem Yong (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Woosuk University) ;
  • Lee Kwang Gyu (Department of Pathology, College of Oriental Medicine, Woosuk University) ;
  • Yu Yun Jo (Department of Physiology, College of Oriental Medicine, Woosuk University) ;
  • Lee Chang Hyun (Department of Anatomy, College of Oriental Medicine, Woosuk University)
  • 발행 : 2002.04.01
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초록

To investigate the anti-inflammatory and analgesic effects of Herba Chelidoniie, the extracts of Herba Chelidoniie treated in arthritic rat model. Complete Freund,s Adjuvant(CFA) were injected in the subcutaneous tissue of left foot paw of rats to induce arthritis. Herba Chelidonii extracts(HC) was administered immediately into the peritoneal cavity after CFA injection for 12 days. The immunohistochemical stainings for calcitonin gene-related peptide(CGRP) and substance P in the L4, L5 and L6 spinal dorsal horn and ganglia were done, and the paw swelling was measured with a micrometer and the blood leukocytes were counted. The results were as follows : The paw swelling of HC treated group was significantly decreased in 12th day after CFA injection compare to control group. The change of differential leukocytes counts of HC treated group increased the ratio of lymphocytes, and decreased the ratio of neutrophils compare to control group. The extent of CGRP immunoreactive nerve fiber of dorsal horn of HC treated group was weakly stained compare to control group. The number of CGRP immunoreactive neurons of L6 spinal cord of HC treated group was significantly decreased compare to control group. The extent of substance P immunoreactive nerve fiber of dorsal horn of He treated group was weakly stained compare to control group. The number of substance P immunoreactive neurons of L4, L5 and L6 spinal cord of HC treated group was significantly decreased compare to control group. These experimental results suggest that Herba Chelidonii extracts reduce the number of CGRP and substance P immunoreactive neurons and nerve fibers of spinal dorsal horns and ganglia, and decrease paw swelling in arthritic rat model, which may be closely related to analgesic and antiinflammatory effects of Herba Chelidonii.

키워드

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