Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Screening of Cytotoxicity and Antimicrobial Effects of Extracts from Atractylodes macrocephala Koidz

백출 추출물의 세포독성과 항균효과검색

  • Choi Eun Young (Department of Beauty and Skin Care, Kimcheon Science College, Kimcheon) ;
  • Oh Hyun Ju (Department of Herbal Resources, Professional Graduate School of Oriental Medicine and Korea Institute of Oriental Pharmacy, Wonkwang University) ;
  • Park Nang Kyu (Department of Herbal Resources, Professional Graduate School of Oriental Medicine and Korea Institute of Oriental Pharmacy, Wonkwang University) ;
  • Chun Hyun Ja (Department of Herbal Resources, Professional Graduate School of Oriental Medicine and Korea Institute of Oriental Pharmacy, Wonkwang University) ;
  • Ahn Jong Woog (Institute of Chemial Research) ;
  • Jeon Byung Hun (Department of Pathology, School of Oriental Medicine, Wonkwang University) ;
  • Han Du Seok (Department of Oral Anatomy, School of Dentistry, Wonkwang University) ;
  • Lee Hyun Ok (Department of Oral Hygiene. Wonkwang Health Science) ;
  • Baek Seung Hwa (Department of Herbal Resources, Professional Graduate School of Oriental Medicine and Korea Institute of Oriental Pharmacy, Wonkwang University)
  • 최은영 (김천과학대학 피부미용과) ;
  • 오현주 (원광대학교 한의학전문대학원 한약자원개발학과, 한국 한약학 연구원) ;
  • 박낭규 (원광대학교 한의학전문대학원 한약자원개발학과, 한국 한약학 연구원) ;
  • 천현자 (원광대학교 한의학전문대학원 한약자원개발학과, 한국 한약학 연구원) ;
  • 안종웅 (한국화학연구원 화학물질연구단) ;
  • 전병훈 (원광대학교 한의과대학 병리학교실) ;
  • 한두석 (원광대학교 치과대학 구강해부학교실) ;
  • 이현옥 (원광보건대학 치위생과) ;
  • 백승화 (원광대학교 한의학전문대학원 한약자원개발학과, 한국 한약학 연구원)
  • Published : 2002.04.01
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Abstract

This study was carried out to evaluate cytotoxic effects of Atractylodes macrocephala Koidz. (A. macrocephala Koidz.) extract on NIH 3T3 fibloblast. SK-MEL-3 (HBT 69) and KB (ATCC No, OCL 17) cell lines. Disruptions in cell organelles were determined by 3-(4,5-dimethylthiazol-2-y1)-2,5-diphenyl-2H-tetrazoliumbromide (MTT) assay. 10.2 mg/ml Concentration of A. macrocephala Koidz. extracts in SK-MEL-3 showed that their susceptibility (sensitivity) to these compounds decreased in the following order ; adriamycin > H₂O > ethyl acetate > ethyl alcohol > chloroform > n-hexane in SK-MEL-3 cell lines ; 5-FU > H₂O > n-hexane > ethyl acetate > ethyl alcohol > chloroform in KB cell lines. In order to develop an antimicrobial agent, A. macrocephala Koidz. was extracted with solvents. The minimal inhibitory concentrations (MICs) of each solvent extract of A. macrocephala Koidz. against microogranisms were also examined. Antimicrobial activities of ampicillin and ketoconazole as references were compared to those of each solvent extract of A. macrocephala Koidz. The antimicrobial activity of the ethyl acetate soluble extract of A. macrocephala Koidz. had growth inhibition activity against S. mutans and P. putida (MICs. 500 ㎍/ml). These results suggest that the ethyl acetate soluble extract of A. macrocephala Koidz. possessed antitumorous and antimicrobial agents

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