Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Effects of Sujeom-san Water Extract in Cultured Rat Myocardial Cells

수념산 전탕액이 배양 심근세포에 미치는 영향

  • Jean Young Seok (Department of Physiology, College of Oriental Medicine, Wonkwang University) ;
  • Kwon Kang Beam (Department of Physiology, College of Oriental Medicine, Wonkwang University) ;
  • Park Eun Young (Department of Physiology, College of Oriental Medicine, Wonkwang University) ;
  • Soong Eun Kyung (Department of Physiology, College of Oriental Medicine, Wonkwang University) ;
  • Park Seung Taeck (Institution of Wonkwang Science, Wonkwang University) ;
  • Ryu Do Gon (Department of Physiology, College of Oriental Medicine, Wonkwang University)
  • 전영석 (원광대학교 한의과대학 생리학교실) ;
  • 권강범 (원광대학교 한의과대학 생리학교실) ;
  • 박은영 (원광대학교 한의과대학 생리학교실) ;
  • 성은경 (원광대학교 한의과대학 생리학교실) ;
  • 박승택 (원광의학연구소) ;
  • 류도곤 (원광대학교 한의과대학 생리학교실)
  • Published : 2002.04.01
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Abstract

To test the protective effect of herbal medicine against oxygen free radical-induced myocardiotoxicity, cytotoxicity of xanthine oxidase/hypoxanthine (XO/HX) was examined using MTT, TBARS, and beating rate assay in the presence of water extract of Sujeom-san(SJS) or single consituents of its prescription. Myocardial toxicity was evaluated in neonatal rat myocardiocytes in cultures. In the present paper, XO/HX resulted in a decrease in viability and beating rate and increases in lipid peroxidation in cultured myocardial cells. In the effect of SJS water extract, it showed effects from the cardiocytotoxicity induced by XO/HX treatment such as increases in beating rate and decreases in lipid peroxidation. In the effect of Rhizoma Corydalis (RC), Faeces Trogopterori (FT), Fructus Amomi Tsaoko (FAT) and Myrrha on the cardiocytotoxicity, they were significantly effective in blocking the XO/HX-induced cardiocytotoxicity by increase of beating rate in FAT and FT group as well as decrease of lipid peroxidation in FT and RC group. These results show that oxygen free radical elicits toxic effects in cultured myocardial cells derived from neonatal rat, and suggest that water extract of Sujeomsan, Rhizoma Corydalis, Faeces Trogopterori, Fructus Amomi Tsaoko or Myrrha is very effective in the prevention of xanthine oxidase/hypoxanthine- induced cardiotoxicity.

Keywords

References

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