Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Protective Effect of Korean Ginseng on Cytotoxicity Induced by 2,2',5,5'-Tetrachlorobiphenyl in Human Neuronal SK-N-MC Cells

환경호르몬 2,2',5,5'-Tetrachlorobiphenyl의 신경세포 독성에 대한 인삼의 방어효과

  • Hwang Sang-Gu (Department of Pathology, College of Oriental Medicine, Wankwang University) ;
  • Kim Ji Su (Department of Pathology, College of Oriental Medicine, Wankwang University) ;
  • Lee Hyung Chul (Department of Pathology, College of Oriental Medicine, Wankwang University) ;
  • Lee Young Chan (Department of Pathology, College of Oriental Medicine, Wankwang University) ;
  • Jeong Young Mok (Department of Pathology, College of Oriental Medicine, Wankwang University) ;
  • Jeong Woo Yeal (Department of Pathology, College of Oriental Medicine, Wankwang University) ;
  • Jeon Byung Hun (Department of Pathology, College of Oriental Medicine, Wankwang University)
  • 황상구 (원광대학교 한의과대학 병리학교실) ;
  • 김지수 (원광대학교 한의과대학 병리학교실) ;
  • 이형철 (원광대학교 한의과대학 병리학교실) ;
  • 이영찬 (원광대학교 한의과대학 병리학교실) ;
  • 정영목 (원광대학교 한의과대학 병리학교실) ;
  • 정우열 (원광대학교 한의과대학 병리학교실) ;
  • 전병훈 (원광대학교 한의과대학 병리학교실)
  • Published : 2002.02.01
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Abstract

Polychlorinated biphenyls(PCBs) are large scale industrial chemicals which are using in diverse applications. The goal of this study was to determine if exposure to 2,2',5,5'-tetrachlorobiphenyl (PCB 52) leads to an increase in the production of active oxidants, and subsequently promotes apoptosis of neuronal SK-N-MC cells. Reactive oxygen species (ROS) formation was examined in SK-N-MC cells after treatment of PCB 52 by concentrations and incubation times, respectively. It showed that the rate of ROS production in the cells was increased in a does-dependent manner to 45 min, followed by a return towards control levels after 120 min treatment. We also examined the association of PCB-induced apoptosis with the modulation of biomakers of oxidative damage to lipids (malondialdehyde [MDA]) in SK-N-MC cells. Increased MDA was observed in a dose-dependent manner in groups treated with 10, 15, and 20 figJ me of PCB 52 for 24 h. After treatment of PCB 52, the cells did not show any significant change in the rate of Cu/Zn-superoxide dismutase (Cu/Zn-SOD) activity. Whereas, the cells had a two-fold greater rate of change in catalase activity at 20 ㎍/㎖ of PCB 52 for 24 h when compared to control group. Korean Ginseng is one of the most important crude drugs which has been used as a traditional Oriental medicine. We next investigated protective effect of extracts of ginseng on cytotoxicity induced by PCB 52 in SK-N-MC cells. Pretreatment of SK-N-MC cells with 25-200 μg/ml of ginseng were reduced cell death in a dose-dependent manner in PCB 52-treated cells. To examine the sensitivity of beta-catenin to ginseng, the protective effect of a range of ginseng concentrations was examined in SK-N-MC cells treated with PCB 52. The result demonstrated that ginseng efficiently blocked PCB 52 inducible beta-catenin proteolysis in a concentration dependent manner. The ROS formation was also measured in the presences of extract of ginseng and superoxide dismutase (inhibitor of oxygen free radical production). The both SOD (400 U/ml) and ginseng (200 μg/ml) significantly inhibited RDS generation in PCB 52-treated group.

Keywords

References

  1. 환경호르몬 이카타 요시토
  2. Crit. Rev. Toxicol. v.24 Polychlorinated biphenyls (PCBs): environmental impact, biochemical and toxic responses, and implications for risk assessment. Safe, S. H. https://doi.org/10.3109/10408449409049308
  3. Regul. Toxicol. Pharmacol. v.21 Human exposure to polychlorinated biphenyls (PCBs): a critical assessment of the evidence for adverse health effects. Swanson, G. M.;Ratcliffe, H. E.;Fischer, L. J. https://doi.org/10.1006/rtph.1995.1018
  4. Environmental Health Series v.29 PCBs, PCDDs, and PCDFs in Breast Milk: assessment of Health Risk. WHO/Europe.
  5. Crit Rev. v.46 Developmental and reproductive toxicity of dioxins and related compounds: cross-species comparisons. Peterson, R. E.;Theobald, M. H.;Kimmel, G. L.
  6. Environ. Health Perspect. v.101 Developmental effects of endocrine-disrupting chemicals in wildlife and humans. Colborn, T.;vom Saal, F. S.;Soto, A. M. https://doi.org/10.2307/3431890
  7. J. Roy. Soc. Health v.111 Oxygen free radicals and human diseases. Aruoma, O. I.;Kaur, H.;Halliwell, B. https://doi.org/10.1177/146642409111100506
  8. Am. J. Clin. Nutr. v.53 Antioxidant nutrient and disease prevention: an over view. Diplock, A. T.
  9. Ann. Neurol. v.32 The oxidant stress hypothesis in Parkinson's disease: evidence supporting it. Fahn, S.;Cohen, G. https://doi.org/10.1002/ana.410320616
  10. Lancet v.344 Free radicals, antioxidants and human disease: curiosity, cause, or consequence? Halliwell, B. https://doi.org/10.1016/S0140-6736(94)92211-X
  11. Clin. Med. J. v.103 Preliminary study on antiamnestic mechanism of ginsenoside Rg1 and Rb1. Zang, J. T.;Qu, Z. W.;Liu, Y.;Deng, H. L.
  12. Proc. 6th Int'l Ginseng korea Ginseng and Tobocco Research Institute Effects of ginsenoside Rb2 on the anti-oxidants in senescence- accelerated mice (SAM-R/1). Chung, Y. H.;Kim, K. W.;Oura, H.
  13. Arch. Toxicol. v.70 Enhanced liver injury in actatalasemic mice following expose to carbontetrachloride. Wang, D. H.;Ishi, K.;Zhen, K.;Taketa, K. https://doi.org/10.1007/s002040050259
  14. Pharm. Biochem. Behav. v.50 Blockade by ginseng total saponin of the development of cocaine induced reverse tolerance and dopamine receptor supersensitivity in mice. Kim, H. S.;Kang, J. K.;Seong, Y. H.;Nam, K. Y.;Oh, K. W. https://doi.org/10.1016/0091-3057(94)00224-7
  15. J. Biol. Chem. v.193 Protein measurment with the folin phenol reagent. Lowry, O. H.;Rosebrough, N. J.;Farr, A. L.;Randall, R. T.
  16. Neurochem. Int. v.17 Sensitive and rapid quantitation of oxygen reactive species formation in rat synaptosomes. LeBel, C. P.;Bondy, S. C. https://doi.org/10.1016/0197-0186(90)90025-O
  17. Clin. Chim. Acta. v.79 Serum lipoperoxide levels in patients suppering from liver disease. Suematsu, T.;Kamada, T.;Abe, H.;Kikuchi, S.;Yagi, K. https://doi.org/10.1016/0009-8981(77)90486-7
  18. J. Biol. Chem. v.231 Superoxide dismutase, Enzymatic function for erythrocuprein (hemocuprein). McCord, J. R.;Colby, M. D.;Fridovich, I.
  19. Verlag Chemie. v.3 Catalase. In methods of enzymatic analysis. Aebi, H.;Bergmeyer, H.;Bergmeyer, J.;Gral, M. (Eds.)
  20. Lab. Invest. v.47 Biology of disease: Free radicals and tissue injury. Freeman, B. A.;Crapo, J. D.
  21. Biochem. Biophys. Res. Commun. v.111 Lipid peroxidation as a possible cause of TCDD toxicity. Stohs, S. J.;Hawwan, M. Q.;Murrau, W. J. https://doi.org/10.1016/0006-291X(83)91377-3
  22. Environ. Health Perspect. v.102 Oxygen-derived species: their relation to human disease and environmental stress. Halliwell, B.;Cross, C. E.
  23. J. Lab. Clin. Med. v.119 Free radicals, antioxidants, and human disease: where are we now. Halliwell, B.;Gutteridge, J. M. C.;Cross, C. E.
  24. J. Toxicol. Environ. Health v.1 Reactive oxygen species and silica-induced carcinogenesis. Shi, X.;Castranova, V.;Halliwell, B.;Vallyathan, V. https://doi.org/10.1080/10937409809524551
  25. Soc. Exp. Biol. Med. v.102 Alterations in intercellular communication during the stage of promotion. Klaunig, J. E.
  26. Chem. Biol. v.2 The role of hydrogen peroxide and superoxide as messengers in the activation of transcription factor NF-KB. Schmidt, K.;Amstad, P.;Ceruti, P.;Baeuerle, P. https://doi.org/10.1016/1074-5521(95)90076-4
  27. J. Clin. Invest. v.89 Low density lipoprotein is protected from oxidation and the progression of atherosclerosis is slowed in cholesterol-fed rabbbits by the antioxidant N,N'-diphenyl-phenylenediamine. Sparrow, C. P.;Doebber, T. W.;Olszewski, J.;Wu, M. S.;Ventre, J.;Stevens, K. A.;Chao, Y. S. https://doi.org/10.1172/JCI115793
  28. Am. J. Physiol. v.266 Reoxygenation injury in isolated ret hepatocytes: Relation to oxygen free radicals and lipid peroxidation. Caraceni, P.;Rosenblum, E. R.;Van, T. D.;Borle, A. B.
  29. FASEB J. v.11 An essential role for free radicals and derived species in signal transduction. Lander, H. M.
  30. Int. J. Vitam. Nutr. Res. v.67 Oxidative stress and signal transduction. Schulze-Osthoff, K.;Bauer, M. K. A.;Vogt, M.;Wasselborg, S.
  31. Oxidative stress: the paradox of aerobic life. Free radicals and Oxidative Stress: Environmental, Drugs, and Food Additives. Davies, K. J. A.;Rice-Evans, C.;Halliwell, B.;Lunt, G. G. (Eds.)
  32. Biochem. v.77 Oxygen free radicals and human disease. Martinez-Cayuela, M. https://doi.org/10.1016/0300-9084(96)88119-3
  33. J. Biol. Chem. v.244 Superoxide dismutase. An enzymic function for erythrocuprein (hemocuprein). McCord, J. M.;Fridovich, I.
  34. J. Biol. Chem. v.248 Mitochondrial superoxide dismutase. Site of synthesis and intramitochondrial location. Weisiger, R. A.;Fridovich, I.
  35. Korean J. Food and Nutrition v.12 Studies on the anti-aging action of Korean ginseng (I): comparative study of red and white ginsengs on anti-aging action. Choi, J. H.;Oh, S. K.
  36. Korean J. Food Sci. Technol. v.14 Studies on the antioxygenic substances in Panax ginseng roots. Paik, T. H.;Hong, J. T.;Hong, S. Y.
  37. J. Korean agricultural chemical society v.26 Effect of solvents on the yield, brown color intensity, UV absorbance, reducing and antioxidant activities of extracts from white and red ginseng. Choi, K.-J.;Kim, M.-W.;Hong, S.-K.;Kim, D.-H.