동의생리병리학회지 (Journal of Physiology & Pathology in Korean Medicine)

  • 제16권1호
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  • Pages.192-196
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  • 2002
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  • 1738-7698(pISSN)
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  • 2288-2529(eISSN)
대한동의생리학회·한의병리학회 ( The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine)
권호 표지

Puerariae radix increases Alcohol-induced Suppressed Cell Proliferation and Expression of Nitric Oxide Synthase in Dentate Gyrus of Rats

  • Jang Mi Hyean (Department of Physiology, College of Medicine, Kyunghee University) ;
  • Lee Taeck Hyun (Department of Physiology, College of Medicine, Kyunghee University) ;
  • Shin Min Chul (Department of Physiology, College of Medicine, Kyunghee University) ;
  • Lim Baek Vin (Department of Physiology, College of Medicine, Kyunghee University) ;
  • Kim Hyun Bae (Department of Physiology, College of Medicine, Kyunghee University) ;
  • Lim Sabina (East-West Medial Research Institute, Kyunghee University) ;
  • Kim Jin Woo (East-West Medial Research Institute, Kyunghee University) ;
  • Lee Cheong Yeal (Department of Physiology, College of Oriental Medicine, Kyungwon University) ;
  • Kim Ee Hwa (Department of Meridianology, College of Oriental Medicine, Semyung University) ;
  • Kim Chang Ju (East-West Medial Research Institute, Kyunghee University)
  • 발행 : 2002.02.01
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초록

Traditionally, aqueous extracts of Puerariae radix had been used for the treatment of alcohol-related problems. In the present study, the effect of Puerariae radix on cell proliferation and expression of nitric oxide synthase (NOS) in the dentate gyrus of alcohol-intoxicated rats were investigated via 5-bromo-2' -deoxyuridine (BrdU) immunohistochemistry and nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-d) histochemistry, respectively. Sprague-Dawley rats weighing 150 ± 10 g were divided into four groups: the control group, the Puerarias radix-treated group, the alcohol-treated group, and the alcohol- and Puerariae radix-treated group. The numbers of both BrdU-positive and NADPH-d-positive cells in the dentate gyrus were inhibited significantly by alcohol administration, while Puerariae radix treatment was shown to increase those numbers. In this study, it was revealed that Puerariae radix possesses protective effect against alcohol-induced suppressed new cell formation and NOS expression in the dentate gyrus. Based on the results, it is possible that NO, which might play an important role in the regulation of cell proliferation, is a major target of the toxic effects of alcohol.

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