The Korean Journal of Physiology and Pharmacology

The Korean Society of Pharmacology (대한약리학회)
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Antagonists of NMDA Receptor, Calcium Channel and Protein Kinase C Potentiate Inhibitory Action of Morphine on Responses of Rat Dorsal Horn Neuron

  • Shin, Hong-Kee (Departments of Physiology, College of Medicine, Hanyang University) ;
  • Kim, Yeon-Suk (Departments of Physiology, College of Medicine, Hanyang University) ;
  • Jun, Jong-Hun (Departments of Anesthesiogy, College of Medicine, Hanyang University) ;
  • Lee, Seo-Eun (Departments of Physiology, College of Medicine, Hanyang University) ;
  • Kim, Jae-Hwa (Department of Orthopaedic Surgery, Bundang Cha Hospital, College of Medicine, Pochun Chu University)
  • Published : 2003.10.21
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Abstract

The present study was designed to examine whether the co-application of morphine with $Ca^{2+}$ channel antagonist $(Mn^{2+},\;verapamil)$, N-methyl-D-aspartate (NMDA) receptor antagonist (2-amino-5-phosphonopentanoic acid$[AP_5]$, $Mg^{2+}$) or protein kinase C inhibitor (H-7) causes the potentiation of morphine-induced antinociceptive action by using an in vivo electrophysiological technique. A single iontophoretic application of morphine or an antagonist alone induced weak inhibition of wide dynamic range (WDR) cell responses to iontophoretically applied NMDA and C-fiber stimulation. Although there was a little difference in the potentiating effects, the antinociceptive action of morphine was potentiated when morphine was iontophoretically applied together with $Mn^{2+}$, verapamil, $AP_5$, $Mg^{2+}$ or H-7. However, the potentiating action between morphine and each antagonist was not apparent, when the antinociceptive action evoked by morphine or the antagonist alone was too strong. These results suggest that the potentiating effect can be caused by the interaction between morphine and each antagonist in the spinal dorsal horn.

Keywords

References

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