The Korean Journal of Physiology and Pharmacology

  • 제7권6호
  • /
  • Pages.363-368
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  • 2003
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  • 1226-4512(pISSN)
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  • 2093-3827(eISSN)
대한약리학회 (The Korean Society of Pharmacology)
권호 표지

Caffeic Acid Phenethyl Ester Inhibits the PKC-Induced IL-6 Gene Expression in the Synoviocytes of Rheumatoid Arthritis Patients

  • Hur, Gang-Min (Department of Pharmacology, College of Medicine, Chungnam National University) ;
  • Hwang, Yin-Bang (Department of Pharmacology, College of Medicine, Chungnam National University) ;
  • Lee, Jae-Heun (Department of Pharmacology, College of Medicine, Chungnam National University) ;
  • Bae, So-Hyun (Department of Pharmacology, College of Medicine, Chungnam National University) ;
  • Park, Ji-Sun (Department of Pharmacology, College of Medicine, Chungnam National University) ;
  • Lee, Choong-Jae (Department of Pharmacology, College of Medicine, Chungnam National University) ;
  • Seok, Jeong-Ho (Department of Pharmacology, College of Medicine,Cancer Research Institute, Chungnam National University)
  • 발행 : 2003.12.21
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초록

To gain insight on the role of pro-inflammatory cytokines in the pathogenesis and treatment of rheumatoid arthritis (RA), the phorbol 12-myristate 13-acetate (PMA)-induced IL-6 gene expression and the effect of caffeic acid phenethyl ester (CAPE) on the PMA-induced IL-6 gene expression were investigated in human fibroblast-like synoviocytes (FLSs). Synovial tissue samples were obtained from rheumatoid arthritis patients, and FLSs were isolated. The cells were stimulated with PMA (100 nM) for 6 hrs to induce IL-6 gene. The cells were pretreated with CAPE (20, 50, $100{\mu}M$) prior to PMA treatment. PMA increased IL-6 RNA expression, binding activities of transcription factors ($NF-{\kappa}B$, AP-1) to IL-6 promoter, and IL-6 promoter activity. However, CAPE inhibited PMA-induced IL-6 mRNA expression in dose-dependent manner, and also inhibited the increased binding activities of transcription factors to IL-6 promoter and IL-6 promoter activity. These results suggest that CAPE might regulate PKC-mediated IL-6 expression and inflammatory reactions in RA.

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