DOI QR코드

DOI QR Code

Effects of Phytase and Carbohydrases Supplementation to Diet with a Partial Replacement of Soybean Meal with Rapeseed Meal and Cottonseed Meal on Growth Performance and Nutrient Digestibility of Growing Pigs

  • Shim, Y.H. (College of Animal Resources Sci., Kangwon National University) ;
  • Chae, B.J. (College of Animal Resources Sci., Kangwon National University) ;
  • Lee, J.H. (EASY BIO System. Inc.)
  • Received : 2003.03.25
  • Accepted : 2003.05.23
  • Published : 2003.09.01

Abstract

An experiment was conducted to investigate the effects of microbial phytase ($Natuphos^{(R)}$) supplementation in combination with carbohydrases (composed of enzymes targeted to soybean meal (SBM) dietary components such as $\alpha$-galactosides and galactomannans; $Endo-Power^{(R)}$) to corn-soybean meal based diet (CSD) and complex diet (CD) with a partial replacement of SBM with rape seed meal (RSM) and cotton seed meal (CSM) on growth performance and nutrient digestibility of growing pigs. A total of 168 growing pigs averaging $13.18{\pm}1.77kg$ of initial body weight was arranged as a $2{\times}2$ factorial design with main effects of diet types (corn-SBM based diet (CSD) and complex diets (CD; 5% of SBM was replaced with 2.5% of RSM and 2.5% of CSM in diet for phase I (0 to 3 weeks) and 6% of SBM was replaced with 3% of RSM and 3% of CSM in diet for phase II (4 to 7 weeks))) and enzyme supplementation (none and 0.1% of phytase (500 FTU/kg diet) and 0.1% of carbohydrases). The diet with enzyme application were formulated to have a 0.18% unit lower aP than diets without enzyme application. Each treatment had three replicates with 14 pigs per replicate. To determine supplementation effect of phytase and carbohydrases on ileal amino acid digestibility of SBM, RSM and CSM, a total of 18 T-cannulated pigs (initial body weight; $13.52{\pm}1.24kg$) were assigned to six dietary treatments in the present study. Dietary treatments in metabolic trial included 1) SBM diet, 2) SBM diet+with enzymes (phytase (500 FTU/kg) and carbohydrases at 0.1%, respectively), 3) CSM diet, 4) CSM diet+enzymes, 5) RSM diet and 6) RSM diet+enzymes. During whole experimental period (0 to 7 wks), there was no difference in growth performance between diets (CSD and CD). However, dietary phytase and carbohydrases supplementation significantly improved gain/feed ratio (G:F) of growing pigs. During the phase II (4-7 weeks), dietary phytase and carbohydrases supplementation significantly improved all fecal nutrient digestibilities (Dry matter (DM), gross energy (GE), crude protein (CP), crude fat (CF), calcium (Ca) and phosphorus (P)). Dietary phytase and carbohydrases supplementation improved significantly overall ileal amino acid digestibilities of SBM, RSM and CSM based diets (p<0.05). The simultaneous inclusion of phytase and carbohydrases in both of CSD and CD reduced feed cost per kg body weight gain (FCG). Also, results suggest that 2.5 to 3% of RSM and CSM, respectively, might be used as a protein source in growing pig diets without having an adverse effect on the growth performance and nutrient digestibility and simultaneous phytase and carbohydrases addition improves nutritional value of SBM, RSM and CSM by improving ileal amino acid digestibilities.

Keywords

References

  1. Aherne, F. X. and J. J. Kennelly. 1985. Oilseed meals for livestock feeding. In Recent Developments in Pig Nutrition, (Ed. D. J. A. Cole and W. Haresign)Butterworths, London, 278.
  2. Aherne, F. X. and J. J. Kennelly. 1983. Oilseed meals for livestock feeding. In: Recent advances in animal nutrition (Ed. W. Haresign). Butterworths. London. p. 3989.
  3. AOAC. 1990. Official Method of Analysis 15th ed., Association of Official Analytical Chemists. Washington, DC.
  4. Bell, J. M. 1984. Nutrients and toxicants in rapeseed meal: a review. J. Anim. Sci. 58:996-1000. https://doi.org/10.2527/jas1984.584996x
  5. Buitrago, J., I. Jimnez, H. Obando, J. H. Maner and A. Moncada 1977. Utilizacin de torta (harina) de algodn en alimentacin del cerdo. Serie ES-25 CIAT, Cali. p. 35.
  6. Caldwell, R. A. 1992. Effect of calcium and phytic acid on the activation of trypsinogen and the stability of trypsin. J. Agric. Food Chem. 40:43-46. https://doi.org/10.1021/jf00013a008
  7. Calloway, D. H., D. J. Colasito and R. D. Mathews. 1966. Gases produced by human intestinal flora. Nature. 212:1238. https://doi.org/10.1038/2121238a0
  8. Coelho, M. B. and E. T. Kornegay. 1996. Phytase in animal nutrition and wate management. BASF Corp., Mount Olive, NJ.
  9. Coon, C. N., K. L. Leske, O. Akavanichan and T. K. Cheng. 1990. Effect of oligosaccharide-free soybean meal on true metabolizable energy and fiber digestion in adult roosters. Poult. Sci. 69:787-793. https://doi.org/10.3382/ps.0690787
  10. Cromwell, G. L., R. D. Coffey, G. R. Parker, H. J. Monegue and J. H. Randolph. 1995. Efficacy of a recombinant-derived phytase in improving the bioavailability of phosphorus in corn-soybean meal diets for pigs. J. Anim. Sci. 73:2000-2008. https://doi.org/10.2527/1995.7372000x
  11. Duncan, D. B. 1955. Multiple range and multiple F test. Biometrics 11:1-42. https://doi.org/10.2307/3001478
  12. Gdala, G. A., J. M. Jansman, L. Buraczewska, J. Huisman and P. van Leeuwen. 1997. The influence of $\alpha$-galactosidase supplementation on the ileal digestibility of lupin seed carbohydrates and dietary protein in young pigs. Anim. Feed Sci. Technol. (67):115-125.
  13. Giesting, D. W. and R. A. Easter. 1991. Effect of protein source and fumaric acid supplementation on apparent ileal digestibility of nutrients by young pigs. J. Anim. Sci. 69:2497-2503. https://doi.org/10.2527/1991.6962497x
  14. Grala, W., M. Verstegen, P. Vanleeuwen, J. Huisman, A. Jansman and S. Tamminga. 1997. Nitrogen balance of pigs as affected by feedstuffs causing different endogenous nitrogen flow at the terminal ileum Livestock-Production-Science. May 1997; 48 (2):143-155. https://doi.org/10.1016/S0301-6226(97)00003-1
  15. Henkel, H. and R. Mosenthin. 1989. Rapssaat und Rapsprodukte in der Tierernhrung. bers. Tierernhrung. 17, S.139-190.
  16. Imbeah, M. and W. C. Sauer. 1991. The effect of dietary level of fat on amino acid digestibilities in soybean meal and canola meal and on rate of passage in growing pigs. Livestock Production Science. 29:227-239. https://doi.org/10.1016/0301-6226(91)90068-2
  17. Jongbloed, A. W. and P. A. Kemme. 1990. Apparent digestible phosphorus in the feeding of pigs in relations to availability, requirementsand environment 1. Digestible phosphorus in feedstuffs from plant and animal origin. Neth. J. Agric. Sci. 38:567-574.
  18. Jongbloed, A. W. 1987. Phosphorus in the feeding of pigs. Ph. D. dissertation, Wageningen, Agricultural University, The Netherlands.
  19. Kim, S. W., I. Mavromichalis and R. A. Easter. 2001a. Supplementation of alpha-1galactosidase and beta-1, 4-mannanase to improve soybean meal utilization by nursery pig. J. Anim. Sci. 79 (Suppl. 1):106 (Abstr.).
  20. Kim, S. W., J. H. Zhang, K. T. Soltwedel and R. A. Easter. 2001b. Supplementation of -1, 6-galactosidase and 1, 4-mannanase to improve soybean meal utilization by growing-finishing pigs. J. Anim. Sci. 79 (Suppl. 2):84 (Abstr.).
  21. Kornegay, E. T. and H. Qian. 1996. Replacement of inorganic phosphorus by microbial phytase for young pigs fed on a maize soyabean meal diet. Br. J. Nutro. 76:563-578. https://doi.org/10.1079/BJN19960063
  22. Kornegay, E. T., J. S. Radcliffe and Z. Zhang. 1998. Influence of phytase and diet composition on phosphorus and amino acid digestibility and phosphorus and nitrogen excretion in swine. In: BASF Tech. Symp., Carolina Swine Nutr. Conf., Durham, NC. pp. 125-155.
  23. De Lange, C. F. M., W. B. Souffrant and W. C. Sauer. 1990. Real ileal protein and amino acid digestibilities in feedstuffs for growing pigs as determined with the 15N-isotope dilution technique. J. Anim. Sci. 68:409-418. https://doi.org/10.2527/1990.682409x
  24. Leske, K. L., C. J. Jevne and C. N. Coon. 1993. Effect of oligosaccharide additions on nitrogen corrected true metabolizable energy of soy protein concentrate. Poult. Sci. 72:664. https://doi.org/10.3382/ps.0720664
  25. Mitaru, B. N., R. Blair, R. D. Reichert, W. E. Roe. 1984. Dark and yellow rapeseed hulls, soybean hulls and a purified fiber source: their effects on dry matter, energy, protein and amino acid digestibilities in cannulated pigs. J. Anim. Sci. Champaign, Ill.: Am. Society Anim. Sci. 59:1510-1518.
  26. Moore, S. 1963. On the determination of cystine as cysteric acid. J. Biol. Sci. 238:235-237.
  27. Mroz, Z. and A. W. Jongbloed. 1998. The influence of phytase on the availability of protein and energy in swine. In: BASF Tech. Symp., Carolina Swine Nutr. Conf., Durham, NC. pp. 65-88.
  28. Mroz, Z., A. W. Jongbloed and P. A. Kemme. 1994. Apparent digestibility and retention of nutrients bound to phytase complexes as influenced by microbial phytase and feeding regimen in pigs. J. Anim. Sci. 72:126-132.
  29. Nair, V. C., J. Laflamme and Z. Duvnjak. 1991. Production of phytase by Aspergillus ficcum and reduction pf phytic acid content in canola meal. J. Sci. Food Agric. 54:355. https://doi.org/10.1002/jsfa.2740540306
  30. NRC. 1998. Nutrient Requirements of Swine (10th Ed.). National Academy Press, Washington DC.
  31. Pettey, L. A., S. D. Carter, B. W. Senne and J. A. Shriver. 2002. Effects of -mannanase addition to corn-soybean meal diets on growth performance, carcass traits and nutrient digestibility of weanling and growing-finishing pigs.
  32. Prawirodigdo, S., E. S. Batterham, L. M. Anderson, F. R. Dunshea and D. J. Farrell. 1997. Nitrogen retention in pigs given diet containing cottonseed meal or soybean meal. Anim. Feed Sci. Technol. 67:205. https://doi.org/10.1016/S0377-8401(96)01116-9
  33. Ravindran, V., P. H. Selle and W. L. Bryden. 1999a. Influence of microbial phytase on apparent ileal amino acid digestibility in feedstuffs for broilers. Poult. Sci. 78:699-706.
  34. Ravindran, V., P. H. Selle and W. L. Bryden. 1999b. Effects of phytase supplementation, individually and in combination, with glucanase on the nutritive value of wheat and barley. Poult. Sci. 78:1588-1595.
  35. SAS. 1985. SAS User's Guide: Statistics, SAS Inst. Inc., Cary, NC.
  36. Sauer, W. C. and P. A. Thacker. 1986. Apparent ileal and faecal digestibility of amino acids in barley-based diets supplemented with soya bean meal or canola meal for growing pigs. Anim. Feed Sci. Tech. 14:183-192. https://doi.org/10.1016/0377-8401(86)90091-X
  37. Selle, P. H., V. Ravindran, G. Ravindran, P. H. Pittolo and W. L. Bryden. 2003. Influence of phytase and xylanase supplementation on growth performance and nutrient utilization of broilers offeredwheat-based diets. Asian-Aust. J. Anim. Sci. Vol 16, No. 3:394-402. https://doi.org/10.5713/ajas.2003.394
  38. Shim Y. H., B. J. Chae and J. H. Lee. 2003. Effects of microbial phytase supplementation in combination with carbohydrases to diets with low nutrient levels on growth performance and ileal nutrient digestibility of weaned pigs. Asian-Aust. J. Anim. Sci. (In press).
  39. Slominski, B. A. and L. D. Campbell. 1990. Non-starch polysaccharides of low-glucosinolate rapeseed (canola) meal: quantification, digestibility in poultry and potential benefit of dietary enzyme supplementation. J. Sci. Food Agric. 53:175-184. https://doi.org/10.1002/jsfa.2740530205
  40. Tanksley Jr., T. D. 1990. Cottonseed meal. In: Non traditional feed sources for use in swine production (Ed. P. A. Thacker and R. N. Kirkwood). Butterworth. Boston, p. 139-151.
  41. Thacker, P. A. 1990. Canola meal. In: Nontraditional feed sources for use in swine production. (Ed. P. A. Thacker and R. N. Kirkwood) Butterworths, Boston, 69.
  42. Veldman, A. V., W. A. G. Veen, D. Barug and P. A. van Paridon. 1993. Effect of -galactosides in feed on ileal piglet digestibility physiology. J. Anim. Nutr. (69):67-65.
  43. Yu, I. T., H. T. Yen and J. F. Wu. 1991. Amino acid additions to low protein corn-soybean meal diets for growing pigs. J. Agric. Res. China. 154:546-554.

Cited by

  1. Effect of phytase supplementation with two levels of phosphorus diets on ileal and faecal digestibilities of nutrients and phosphorus, calcium, nitrogen and energy balances in growing pigs vol.81, pp.01, 2005, https://doi.org/10.1079/ASC41680067
  2. Enzimas carboidrases na dieta de porcas lactantes e suas leitegadas vol.39, pp.9, 2009, https://doi.org/10.1590/S0103-84782009000900030
  3. Effects of corn dried distiller's grains with solubles and enzyme premix supplements on growth performance, carcass characteristics and meat quality parameters in finishing pigs vol.82, pp.3, 2011, https://doi.org/10.1111/j.1740-0929.2010.00848.x
  4. Effects of exogenous enzyme supplementation to corn- and soybean meal-based or complex diets on growth performance, nutrient digestibility, and blood metabolites in growing pigs1 vol.90, pp.9, 2012, https://doi.org/10.2527/jas.2010-3430
  5. Effects of Eco-friendly Multi-enzyme on Growth Performance, Intestinal Morphology, and Nutrient Digestibility of weaned Pigs vol.26, pp.1, 2018, https://doi.org/10.11625/KJOA.2018.26.1.141
  6. Effects of Enzyme Addition to Broiler Diets Containing Varying Levels of Double Zero Rapeseed Meal vol.19, pp.9, 2003, https://doi.org/10.5713/ajas.2006.1354