Animal cells and systems

The Korean Society for Integrative Biology (한국통합생물학회)
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Promoter Structure and Transcriptional Activity of Human Complement Receptor Type I (CR1) Gene

  • Kim, Jae-Hyun (Department of Biology, School of Life Sciences, Research Center for Bioresource and Health, Chungbuk National University) ;
  • Lee, Young-Ju (Department of Biology, School of Life Sciences, Research Center for Bioresource and Health, Chungbuk National University) ;
  • Nam, Ju-Ryoung (Department of Biology, School of Life Sciences, Research Center for Bioresource and Health, Chungbuk National University) ;
  • Shim, Hee-Bo (Department of Biology, School of Life Sciences, Research Center for Bioresource and Health, Chungbuk National University) ;
  • Choe, Soo-Young (Department of Biology, School of Life Sciences, Research Center for Bioresource and Health, Chungbuk National University)
  • Published : 2003.03.01
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Abstract

Until recently, interest in human complement receptor type I (CR1) has focused on immune complex processing, which contributed to our understanding of regulatory mechanism of complement activation. However, the promoter structure and transcriptional regulation of human CR1 gene has not been clear. To study the unique regulation of human CR1 gene expression, we assessed promoter activity of the $5^1$-flanking region of human CR1 gene using transient transfection and gel mobility shift assays. In this study we demonstrated that NF-Y binds to the inverted CCAAT element and that the functional interaction with protein(s) which bind to the GC-rich motif may be necessary for optimal transcription of human CR1 gene. We also show that sequence elements which located at-95/58 and +45/+50 are important for optimal transcription of CR1 gene.

Keywords

References

  1. Chodosh LA, Baldwin AS, Carthew RW, and Sharp PA (1988) Hurran CCAAT-binding proteins have heterologous subunits. Cell 53: 11-24 https://doi.org/10.1016/0092-8674(88)90483-7
  2. Dorn A, Bollekens J, Staub A, Benoist C, and Mathis, D (1987) A multiplicity of CCAAT box-binding proteins. Cell 50: 863-872 https://doi.org/10.1016/0092-8674(87)90513-7
  3. Fearon DT (1980) Identification of the membrane glycoprotein that is the C3b receptor of the human erythrocyte, polymorphonuclear leukocyte, B lymphocyte, and monocyte. J Exp Med 152: 20-30 https://doi.org/10.1084/jem.152.1.20
  4. Fearon DT and Wong WW (1983) Complement ligand-receptor interactions that mediated biological responses. Annu Rev Immunol 1: 243-271 https://doi.org/10.1146/annurev.iy.01.040183.001331
  5. Funkhouser T and Vik DP (2000) Promoter activity of the 5' flanking region of the complement receptor type 1 (CR1) gene: basal and induced transcription. Biochem Biophys Acta 1490: 99-105 https://doi.org/10.1016/S0167-4781(99)00245-6
  6. Gelfand MC, Shin ML, Nagle RB, Green I, and Frank MM (1976) The glomerular complement receptor in immunologically mediated renal glomerular injury. New Engl J Med 295: 10-14 https://doi.org/10.1056/NEJM197607012950103
  7. Hooft van Huijsduijnen RA, Bollekens J, Dorn A, Benoist C, and Mathis D (1987) Properties of a CCAAT box-binding protein. Nucleic Acids Res 15: 7265-7282 https://doi.org/10.1093/nar/15.18.7265
  8. Kazatchkine MD, Fearon DT, Appay M, Mandet DG, and Bariety J (1982) Immunohistochemical study of the human glomerular C3b receptor in normal kidney and in seventy five cases of renal diseases: loss of c3b receptor antigen in focal hyalinosis and in proliferative nephritis of systemic lupus erythematosus. J Clin Invest 69: 900-912 https://doi.org/10.1172/JCI110529
  9. Kim IS, Sinha S, de Crombrugghe B, and Maity SN (1996) Determination of functional domains in the C subunit of the CCAAT-binding factor (CBF) necessary for formation of a CBF-DNA complex: CBF-B interacts simultaneously with both the CBF-A and CBF-C subunits to form a heterotrimeric CBF molecule. Mol Cell Biol 16: 4003-4013 https://doi.org/10.1128/MCB.16.8.4003
  10. Kim JH, Lee S, and Choe SY (1999a) Characterization of the human CR1 gene promoter. Biochem Mol Biol Int 47: 655-663
  11. Kim JH, Lee S, Rho JK, and Choe SY (1999b) AML1, the target of chromosomal rearrangements in human leukemia, regulates the expression of human complement receptor type 1 (CR1) gene. Int J Biochem Cell Biol 31: 933-940 https://doi.org/10.1016/S1357-2725(99)00048-5
  12. Lublin DM, Lemons RS, Le Beau MM, Holers VM, Tykocinski ML, Medof ME, and Atkinson JP (1987) The gene encoding decay accelerating factor (DAF) is located in the complement-regulatory locus on the long arm of chromosome 1, J Exp Med 165: 1731-1736 https://doi.org/10.1084/jem.165.6.1731
  13. Maity SN, Vuorio T, and de Crombrugghe B (1990) The B subunit of a rat heteromeric CCAAT-binding transcription factor shows a striking sequence identity with the yeast Hap2 transcription factor. Proc Natl Acad Sci USA 87: 5378-5382 https://doi.org/10.1073/pnas.87.14.5378
  14. Pahl HL, Scheibe RJ, Zhang DE, Chen HM, Galson DL, Maki RA, and Tenen DG (1993) The proto-oncogene PU.1 regulates expression of the myeloid-specific CD11b promoter. J Biol Chem 268: 5014-5020
  15. Reynes M, Aubert JP, Cohen JHM, Audouin J, Tricotet V, Diebold J, and Kazatchkine MD (1985) Human follicular cells express CR1, CR2 and CR3 complement receptor antigens. J Immunol 135: 2687-2694
  16. Rho JK, Kim JH, Yu J, and Choe SY (2002) Correlation between cellular localization of TEL/AML1 fusion protein and repression of AML1-mediated transactivation of CR1 gene. Biochem Biophys Res Commun 297: 91-95 https://doi.org/10.1016/S0006-291X(02)02075-2
  17. Roder K, Wolf SS, Beck K -F, and Schweizer M (1997) Cooperative binding of NF-Y and Sp1 at the DNase I-hypersensitive site, fatty acid synthase insulin-responsive element 1, located at -500 in the rat fatty acid synthase promoter. J Biol Chem 272: 21616-21624 https://doi.org/10.1074/jbc.272.34.21616
  18. Roder K, Wolf SS, Larkin KJ, and Schweizer M (1999) Interaction between the two ubiquitously expressed transcription factors NF-Y and Sp1. Gene 234: 61-69 https://doi.org/10.1016/S0378-1119(99)00180-8
  19. Rodriguez de Cordoba S, Lubin D, Rubinstein P, and Atkinson JP (1985) Human genes for three complement components that regulate the activation of C3 are tightly linked. J Exp Med 161: 1189-1195 https://doi.org/10.1084/jem.161.5.1189
  20. Ross GD and Medof ME (1985) Membrane complement receptors specific for bound of C3. Adv Immunol 37: 217-267 https://doi.org/10.1016/S0065-2776(08)60341-7
  21. Schreiber E, Matthias P, Muller MM, and Shaffner W (1989) Rapid detection of octamer binding proteins with 'mini-extracts', prepared from a small number of cells. Nucleic Acids Res 17: 6419 https://doi.org/10.1093/nar/17.15.6419
  22. Sinha S, Maity SN, Lu J, and de Crombrugghe B (1995) Recombinant rat CBF-C, the third subunit of CBF/NFY, allows formation of a protein-DNA complex with CBF-A and CBF-B and with yeast HAP2 and HAP3. Proc Natl Acad Sci USA 92: 1624-1628 https://doi.org/10.1073/pnas.92.5.1624
  23. Vuorio T, Maity SN, and de Crombrugghe B (1990) Purification and molecular cloning of the 'A' chain of a rat heteromeric CCAAT-binding protein: Sequence identity with the yeast HAP3 transcription factor. J Biol Chem 265: 22480-22486
  24. Weis JH, Morton CC, Bruns GA, Weis JJ, Klickstein LB, Wong WW, and Fearon DT (1987) A complement receptor locus: genes encoding C3b/C4b receptor and C3d/Epstein-Barr virus receptor map to 1q32. J Immunol 138: 312-315
  25. Wilson JG, Tedder TF, and Fearon DT (1983) Characterization of human T lymphocytes that express the C3b receptor. J Immunol 131: 684-689