Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Gene Expression Analyses in Hypothalami of Immobilization-stressed and BoshimgeonbiTang-treated Mice Using cDNA Microarray

구속 스트레스 (immobilization stress)를 가한 rat의 hypothalamus에서의 유전자 발현 및 포심건비탕의 항스트레스 효과에 관한 cDNA microarray 분석

  • Lee Han Chang (Department of Oriental Medical Science, Graduate School of East-West Medical Science, Kyunghee University) ;
  • Yeam Mi Jung (Department of Oriental Medical Science, Graduate School of East-West Medical Science, Kyunghee University) ;
  • Kim Gun Ho (Department of Oriental Medical Science, Graduate School of East-West Medical Science, Kyunghee University) ;
  • Choi Kang Duk (East-West Kidney Center, Kyunghee Medical Center) ;
  • Lee Seoung Hee (Department of Pediatrics, College of Oriental Medicine, Dogguk University) ;
  • Shim Insop (Department of Oriental Medical Science, Graduate School of East-West Medical Science, Kyunghee University) ;
  • Lee Hye Jung (Department of Oriental Medical Science, Graduate School of East-West Medical Science, Kyunghee University) ;
  • Hahm Dae Hyun (Department of Oriental Medical Science, Graduate School of East-West Medical Science, Kyunghee University)
  • 이한창 (경희대학교 동서의학대학원) ;
  • 염미정 (경희대학교 동서의학대학원) ;
  • 김건호 (경희대학교 동서의학대학원) ;
  • 최강덕 (경희대학교 의과대학 동서신장센터) ;
  • 이승희 (동국대학교 한방병원 소아과) ;
  • 심인섭 (경희대학교 동서의학대학원) ;
  • 이혜정 (경희대학교 동서의학대학원) ;
  • 함대현 (경희대학교 동서의학대학원)
  • Published : 2003.12.01
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Abstract

The genetic effects of restraint stress challenge on HPA axis and the therapeutic effect of Boshimgeonbi-Tang on the stress were studied with cDNA microarray analyses on hypothalamus using an immobilization-stress mouse as stress model. Male CD-1 mice were restrained in a tightly fitted and ventilated vinyl holder for 2hours once a day, and this challenge was repeated for seven consecutive days. The body weights of the immobilization-stress mice were diminished about 25 percent degree as compared to normal ones. Seven days later, total RNA was extracted from the organs of the mouse, body-labeled with CyDye/sup TM/ fluorescence dyes (Amersham Bioscience Co., NJ), and then hybridized to cDNA microarray chip. Scanning and analyzing the array slides were carried out using GenePix 4000 series scanner and GenePix Pro/sup TM/ analyzing program, respectively. The expression profiles of 109 genes out of 6000 genes on the chip were significantly modulated in hypothalamus by the immobilization stress. Energy metabolism-, lipid metabolism-, apoptosis- and signal transduction-related genes were transcriptionally activated whereas DNA repair-, protein biosynthesis-, and structure integrity-related genes were down-regulated in hypothalamus. The 58 genes were up-regulated by the mRNA expression folds of 1.5 to 7.9. and the 51 genes were down-regulated by 1.5 - 3.5 fold. The 20 genes among them were selected to confirm the expression profiles by RT-PCR. The mRNA expression levels of Tnfrsf1a (apoptosis), Calm2 (cell cycle), Bag3 (apoptosis), Hspe1 (protein folding), Aatk (apoptosis), Dffa (apoptosis), Itgb1 (cell adhesion), Vcam1 (cell adhesion), Fkbp5 (protein folding), BDNF (neuron survival) were restored to the normal one by the treatment of Boshimgeonbi-Tang.

Keywords

References

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