오배자가 구강에서 분리된 미생물에 대한 항균효과 및 구강 편평세포암종 KB 세포에 미치는 영향

Antimicrobial and Anticancer Effects of Galla Rhois on Pathogens Isolated from Oral and KB Human Oral Epidermoid Carcinoma Cells

  • 이영선 ((재)경북테크노파크, 대구한의대학교 한방생명자원특화센터 효능검증원) ;
  • 한옥경 ((재)경북테크노파크, 대구한의대학교 한방생명자원특화센터 효능검증원) ;
  • 배만종 ((재)경북테크노파크, 대구한의대학교 한방생명자원특화센터 효능검증원) ;
  • 김광중 ((재)경북테크노파크, 대구한의대학교 한방생명자원특화센터 효능검증원) ;
  • 신상우 (대구한의대학교 한의학과 병리학교실) ;
  • 이송권 (대구한의대학교 보건대학원) ;
  • 박종현 (대구한의대학교 한의학과 병리학교실)
  • Lee Young Sun (Efficacy and Safety Research Center for Traditional Oriental Medicine, Daegu Hanny University, Kyongbuk Technopark) ;
  • Han Ok Kyung (Efficacy and Safety Research Center for Traditional Oriental Medicine, Daegu Hanny University, Kyongbuk Technopark) ;
  • Bae Man Jong (Efficacy and Safety Research Center for Traditional Oriental Medicine, Daegu Hanny University, Kyongbuk Technopark) ;
  • Kim Kwang Joong (Efficacy and Safety Research Center for Traditional Oriental Medicine, Daegu Hanny University, Kyongbuk Technopark) ;
  • Shin Sang Woo (Department Pathology, College of Oriental Medicine, Daegu Hanny University) ;
  • Lee Song Kwon (Graduate School of Public Health, Daegu Hanny University) ;
  • Park Jong-Hyun (Department Pathology, College of Oriental Medicine, Daegu Hanny University)
  • 발행 : 2003.12.01

초록

This study was carried out to investigate the antimicrobial and anticancer effects of Galla Rhois (GR) on pathogens isolated from oral and KB human oral epidermoid carcinoma cells. Their antimicrobial activities were tested against Streptococcus mutans (S. mutans), Staphylococcus aureus (S. aureus), Enterobacter aerogenes (E. aerogenes), Escherichia coli (E. coil) and Candida albicans (C. albicans). GR powder has the antimicrobial activity against C. albicans, S. mutans and S. aureus. The extracts of water and ethanol have the antimicrobial activity against S. sureus and C. albicans. The water extract showed inhibitory activity against the growth and pH of above mentioned reference microorganisms. The water extract of GR declined cell viability in a dose dependent manner. DNA flow cytometric analysis showed that population of sub-G/sub 0//G₁, phase of cell cycle was increased by GR extract treatment in a dose dependent manner. Western blot analysis revealed that Caspase-3 was reduced by GR extract treatment. These result suggested that GR has the effect of antimicrobial on pathogens isolated from oral, and also, has anticancer effect that associated with the induction of apoptosis in a dose dependent manner in KB human oral epidermoid carcinoma cells. It may be GR-induced apoptosis was mediated via activation of Caspase-3.

키워드

참고문헌

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  2. 한국위생과학회지 v.4 구강균의 생육에 대한 각종 향신료의 영향 김지화;송경희;윤수홍
  3. 한국수산학회지 v.35 충치균(Streptococcus mutans)에 대한 다시마 추출물의 항균활성 김지회;이두석;임치원;박희연;박정흠
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