Effects of Mahwangyoonpye-tang on Asthma Induced by Ovalbumin in Mouse

마황윤폐탕이 ovalbumin으로 유발된 mouse의 천식에 미치는 영향

  • Kim Ji Yun (Department of Oriental Medicine, Graduate School, Dongeui University) ;
  • Park Dong il (Department of Oriental Medicine, Graduate School, Dongeui University) ;
  • Kim Jong Dae (Department of Oriental Medicine, Graduate School, Daegu University) ;
  • Kam Cheol Woo (Department of Oriental Medicine, Graduate School, Dongeui University)
  • 김지윤 (동의대학교 한의과대학 폐계내과학교실) ;
  • 박동일 (동의대학교 한의과대학 폐계내과학교실) ;
  • 김종대 (대구대학교 한의과대학 폐계내과학교실) ;
  • 감철우 (동의대학교 한의과대학 폐계내과학교실)
  • Published : 2003.12.01

Abstract

In order to study the effect of oral administration of Mawhangyounpye-tang against to asthma, astham was induced to allergy-sensitive Balb/c mouse with ovalbumin using method of Hatfield et al (1997). The changes of diameter lumen of upper portion of the trachea, lung weight, gross appearance of lung, histological profiles of lung and trachea, numbers of cellular compartments in the bronchoalveolar lavage fluid (BALF), numbers and morphology of the mast cells in the trachea, numbers of mucus-secretory cell in the broncus, morphology of the bronchus, ultramicroscopical appearance of surface of trachea and number of cilia and mucous-secretory cells by scanning electron microscope. Obtained results were as follows. 1. The diameters of trachea lumen were significantly decreased in asthma induced control groups and these decreasing were result from hypertrophy of mucous membrane. However, these phenomena were dramatically recovered in the Mawhangyounpye-tang dosing groups. 2. Lung weights and black spots, which were result from infiltration of inflammatory cells, were significantly increased in asthma induced control groups but these phenomena were dramatically recovered in the Mawhangyounpye-tang dosing groups. 3. Hypertrophy of mucous membrane of trachea and bronchus and !bronchioles in the lung, peritracheal, peribronchus and peribronchiolar inflammatory cell infiltration, and mucoid exudate deposit in the lumen were observed in asthma induced control groups but these phenomena were dramatically recovered in the Mawhangyounpye-tang dosing groups. 4. Cellular compartments including neutrophil and eosinophil were dramatically increased in the BALF of asthma induced control groups but these phenomena were dramatically recovered in the Mawhangyounpye-tang dosing groups. 5. Mast cell degranulation and decreasing of the numbers of mast cells were detected in the trachea of asthma induced control groups. However, these phenomena were dramatically recovered in the Mawhangyounpye-tang dosing groups. 6. Shed, decreasing of cilia cell and increasing of mucous-secretory cells in the surface of the trachea of asthma induced control groups but these phenomena were dramatically recovered in the Mawhangyounpye-tang dosing groups. In conclusion, it Is considered that Mawhangyounpye-tang has somewhat favorable effect on the asthma because the asthma specific series of abnormalities in respiratory system were decreased after oral administratin of Mawhangyounpye-tang in this study. In future, it is needed that the toxicological and dosagespecific study of Mawhangyounpye-tang to use against asthma with safe.

Keywords

References

  1. 해리슨 내과학(한글1) 해리슨내번역편찬위원회
  2. 解表二陣湯加減方이 喘息 모델 내의 Cytokine에 미치는 影響 백동진;정희재;이형구;정승기
  3. 巢氏諸病源候論(卷13, 14) 巢元方
  4. 醫門寶鑑 周命新
  5. 臨證指南醫案 葉天士
  6. 編註醫學入門 李천
  7. 景岳全書 張介賓
  8. 韓方臨床病理 北京中醫學院(編)
  9. 한방기본처방집 경산대부속한방병원
  10. Ovalbumin에 의한 기니픽 천식 유발시 폐의 조직 병리학적 변화에 미치는 상백피의 영향 소병준
  11. 대한면역학회지 v.16 기니픽의 후기 천식반응에 미치는 상백피의 억제 효과 이무삼;강경진;채옥희;김봉천;정순용;이양근
  12. 小靑龍湯이 알레르기 喘息 모델 흰쥐의 BALF내 免疫細胞에 미치는 影響 李俊雨
  13. 麥門冬湯과 定喘化痰降氣湯이 알레르기 喘息 모델 흰쥐의 BALF內 免疫細胞 및 血淸 IgE에 미치는 影響 金珍珠
  14. 瀉白散이 喘息에 미치는 效能에 關한 分子生物學的硏究 趙一賢
  15. 慶熙韓醫大論文集 v.22 no.1 淸上補下湯이 Allergy喘息의 呼吸樣狀과 氣管粘膜의 好酸球變化에 미치는 影響 李珩九;鄭昇杞;鄭熙才;權赫星
  16. 黃帝內經素問譯解 楊維傑
  17. 丹溪心法 朱震亨
  18. 證治準繩 王肯堂
  19. 醫學正傳 虞搏
  20. 醫宗金鑑 吳謙
  21. 中醫內科學 上海中醫學院
  22. 東醫寶鑑 許浚
  23. J Allergy Clin Immunol v.102 Effects of disel exhaust on allergic airway inflammation in mice Miyabara, Y.;Ichinose, T.;Takano, H.;Lim, H.B.;Sagaim M.
  24. Eur J Pharmacol v.293 Bronchoconstriction and airway hyperresponsiveness after ovalbumin inhalation in sensitized mice Hessel, E.M.;Van Oosterhout, A.J.;Hofsta, C.L.;De Bie, J.J.; Garssen, J.;Van Loveren, H.;Verheyen, A.K.;Savelkoul, H.F.;Nijkamp, F.P.
  25. Respiration v.65 Effects of inhaled cyclosporin on the rat air way: histologic and bronchoalveolar lavage assessment Ceyhan, Y.;Sungur, M.;Celikel, C.A.;Ceilikel, T.
  26. Arerugi v.44 Inhibitory effect of low molecular weight heparin on the bronchoconstriction in ovalbumin-sensitized quinea pigs after antigen exposure Maeda, H.;Yahata, T.;Satouchi, M.;Takenaka, K.;Yokoyama, M.
  27. Am J Respir Cell Mol Biol v.13 Effect of dexamethasone on antigen-induced high molecular weight glycoconjugate secretionin allergic guinea pigs Savoie, C.;Plant, M.;Zwikker, M.;Van Staden, C.J.;Boulet, L.;Chan, C.C.;Rodger, I.W.;Pon, D.J.
  28. 한이인지 v.39 알레르기가 유발된 기늬픽 비점막에서 호산구 침윤과 상피 손상과의 관계 홍순관
  29. Clin Exp Allergy v.25 Surfactant dysfunction develops when the immunized quinea-pig is challenged with ovalbumin aerosol Liu, M.;Wang, L.;Enhorning, G.
  30. Eur J Pharmacol v.293 Bronchoconstriction and airway hyperres- ponsiveness after ovalbumin inhalation in sensitized mice Hessel, E.M.;Van Oosterhout, A.J.;Hofsta, C.L.;De Bie, J.J.; Garssen, J.;Van Loveren H.;Verheyen, A.K.;Savelkoul, H.F.;Nijkampm F.P.
  31. Am Rev Respir Dis v.137 Development of a rolonged eosinophil-rich inflammatory leukocyte infiltration in the quinea pig asthmatic response to ovalbumine inhalation Dunn, C.J.;Elliot, G.A.;OOsteven, J.A.;Richard, I.M.
  32. Clin Exp Allergy v.28 Leflunomide, a novel immunomodulating agent, prevents the development of allergic sensitization in an animal model of allergic asthma Eber, E.;Uhlig, T.;McMenamin, C.;Sly, P.D.
  33. J Pharmacol Exp Ther v.280 The influence of endogenous catecholamines on the inhibitory effects of rolipram against early- and late-phase response to antigen in the guinea pig Underwood, D.C.;Matthews, J.K.;Osborn, R.R.;Bochnowicz, S.;Torphy, T.J.
  34. J Appl Physiol v.39 Pulmonary response to antigen infusion in the sensitized quinea pig: modification by atropine Drazen, J.M.;Austen, K.F.
  35. Endocrinology v.138 Relaxin ciunteracts asthma-like reaction induced antigen in sensitized guinea pigs Bani, D.;Ballati, L.;Masini, E.;Bigazzi, M.;Sacchi, T.B.
  36. Pneumonol Alergol Pol v.63 Histology of the respiratory system after exposure to bacterial infection and formalin vapors used to induce experimental bronchospasm Sachs, K.;Pindel, B.;Jastrzab, J.;Gawlik, R.;Rogala, E.
  37. Am Rev Respir Dis v.137 Early and late-phase bronchoconstriction after allergen challenge of nonanesthetized guinea pigs. Ⅰ. The association of disordered airway physiology to leukocyte infiltration Huston, P.A.;Church, M.K.;Clay, T.P.;Miller, P.;Holgate, S.T.
  38. Nihon Kokyuki Gakkai Zasshi v.36 Effect of heparin on airway goblet cell secretion in sensitized guinea pigs Nakata, J.;Tamaoki, J.;Takeyama, K.;Takeda, Y.;Yamakawa, I.;Kondo, M.;Nagai, A.
  39. Am J Respir Cell Mol Biol v.14 Lung inflammation and epithelial chages in a murine model of atopic asthma Blyth, D.I.;Pedrick, M.S.;Savage, T.J.;Hessel, E.M.;Fattah, D.
  40. Eur Respir J v.1 Morphological studies of bronchial mucosal biopsies from asthmatics before and after ten years of treatment with inhaled steroids Lundgren, R.;Soderberg, M.;Horstedt, P.;Stenling, R.