Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Comparison of Relative Antioxidant Capacity and Antiperoxidation Activity of Traditional Medicines in Vitro

  • Yeom Gee Bok (Department of Oriental Pathology, College of Oriental Medicine, Wonkwang University) ;
  • Lee Hyung Cheol (Department of Oriental Pathology, College of Oriental Medicine, Wonkwang University) ;
  • Ju Sung Min (Department of Oriental Pathology, College of Oriental Medicine, Wonkwang University) ;
  • Kim Kun Jung (Department of Oriental Pathology, College of Oriental Medicine, Wonkwang University) ;
  • Kim Won Sin (Division of Life science College of Natural Sciences and Institute of Basic Natural Sciences, Wonkwang University) ;
  • Lee Chae Ho (Division of Life science College of Natural Sciences and Institute of Basic Natural Sciences, Wonkwang University) ;
  • Jang Ho Yeon (Department of Oriental Pathology, College of Oriental Medicine, Wonkwang University) ;
  • Kang Jeong Ho (Department of Oriental Pathology, College of Oriental Medicine, Wonkwang University) ;
  • Park Shin Ki (Department of Oriental Pathology, College of Oriental Medicine, Wonkwang University) ;
  • Lee Key Sang (Department of Oriental Pathology, College of Oriental Medicine, Wonkwang University) ;
  • Jeon Byung Hun (Department of Oriental Pathology, College of Oriental Medicine, Wonkwang University)
  • Published : 2003.12.01
Download PDF
⟨ Previous Next ⟩

Abstract

To investigate the antioxidant capacity of traditional Korean medicines, water extracts from 57 species were tested on their antioxidant activity using radical scavenging effects against ABTSㆍ+. Of which, Rhus javanica, Caesalpinia sappan, Rosa rugosa, Spatholobus suberectus, and Magnolia obovata showed strong antioxidant capacities at 10㎍ concentration. Therefore, antioxidant capacities of 5 traditional medicine extracts in the different concentration (1㎍, 5㎍, and 10㎍) were determined. The 5 traditional medicine extracts was detected in antioxidant capacity dose dependently. R. javanica was showed the highest antioxidant capacity, the antioxidant activity at 1 ㎍ of herbal extract being 0.85 mM TE. At the same time, the antiperoxidation effects of these 5 medicines were determined. Lipid peroxidation in brain homogenates induced by NADPH and ADP-Fe/sup 2+/ was strong inhibited by S, suberectus, R. rugosa, and M. obovata. Extract of C. sappan was showed the highest inhibition against lipid peroxidation compared with 4 herbal extracts in vitro. These traditional medicines are a potent antioxidant capacity and antiperoxidation activity, further investigation into the in vivo antioxidant therapeutic potential for treatment of human disorders such as Alzheimer's disease.

Keywords

References

  1. Lancet v.344 Oxy-radicals and cancer Cerutti, P.A.
  2. J. Ethnopharmacol. v.77 The in vitro effect of Helichrysi flos on microsomal lipid peroxidation Czinner, E.;Hagymasi, K.;Blazovics;Kery, A.;Szoke, E.;Lemberkovics, E.
  3. Lancet v.347 Oxidative damage to DNA in diabetes mellitus Dnadona, P.;Thusu, K.;Cook, S.; Snyder, B.;Makowski, J.;Armstrong, D.;Nicotera. T.
  4. Neurotoxicol. Teratol. v.22 Age-related changes in reactive oxygen species production in rat brain homogenates Driver, A.S.;Kodavanti, P.R.S.;Mundy, W.R.
  5. Bull. world Health Organ. v.63 Medicinal plants in therapy Farnsworth, N.R.;Akerele, O.;Bingel, A.S.;Soejarto, S.S.;Guo, Z.
  6. Pharmacol. Thr. v.63 Oxidative stress: free radical production in neural degeneration Gotz, M.E.;Kunig, G.;Riederer, P.;Youdim, M.B.
  7. Gen. Pharmacol. v.32 Free-radical scavenging action of medicinal herbs from Ghana Thonningia sanguinea on experimentally-induced liver injuries Gyamfi, M.A.;Yonamine, M.;Aniya, Y.
  8. Meth. Enzymol. v.186 Role of free radicals and catalytic metal ions in human disease: an overview Halliwell, B.;Gutteridge, J.M.C.
  9. Phytochem. v.26 Hydroxyl radical scavenging activity of flavonoids Husain, S.R.;Cillard, J.;Cillard, P.
  10. Zeitschrift Fur Naturforschung Section C-A J. Biosci. v.54 Protective effect of quaternary piperidinium salts on lipid oxidation in the erythrocyte membrane Kleszcynska, H.;Oswiecimska, M.;Sarapuk, J.;Witek, S.;Przestalski, S.
  11. Kor. J.Pharm. v.28 Screening of the acetylcholinesterase inhibitors from medicinal plants Lee, B.H.;Choi, B.W.;Ryu, G.S.;Lee, E.S.;Kang, K.J.;Hwang, D.Y.; Hong, N.D.
  12. Clin. Chim. Acta v.301 Comparative antioxidant potential of anaethetics and perioperative drugs in vitro Mantle, D.;Eddeb, F.;Areni, K.;Snowden, C.;Mendelow, A.D.
  13. J. Ethnopharmacol. v.72 Comparison of relative antioxidant activities of British medicinal plant species in vitro Mantle, D.;Eddeb, F.;Pickering, A.T.
  14. Biochem. Pharmacol. v.15 NADPH-dependent and O2-dependent lipid-peroxidation Nizamuddin, A.
  15. J. Cereal Sci. v.33 Oat antioxidants. Peterson, D.M.
  16. Food Sci. Tech. Int. v.6 The contribution of plant food antioxidants to human health Ramarathnam, N.;Osawa, T.;Ochi, H.;Kawakishi, S.
  17. Meth. Enzymol. v.234 Total antioxidant status in plasma and body fluids Rice-Evans, C.;Patterson, N.J.
  18. Ann. Clin. Biochem. v.35 Analytical aspects of antioxidants and free radical activity in clinical biochemistry Rumley, A.G.;Patterson, J.R.
  19. Toxicol. Appl. Pharmacol. v.78 Organic hydroperoxide-induced lipid peroxidation and cell death in isolated hepatocytes Rush, G.F.;Gorski, J.R.;Ripple, M.G.;Sowinski, J.;Bugelski, P.;Hewitt, W.R.
  20. Technical Briefs v.42 Shortcomings of an automated assay for total antioxidant status in biological fluids Schofield, D.;Braganza, J.M.
  21. Free Rad. Biol. Med. v.9 Metal ion-catalyzed oxidation of proteins: biochemical mechanisms and biological consequences Stadtman, E.R.
  22. Biosic. Biotech. Biochem. v.58 A simple screening method for antioxidants and isolation of several antioxidants produced by marine bacteria from fish and shellfish Takao, T.;Kitatani, F.;Watanabe, N.;Yagi, A.;Sakata, K.
  23. J. Environ. Health v.C8 Effective human cancer prevention as goal for the regulation of environmental chemicals Weisburger, J.H.
  24. Food Chem. Toxicol. v.37 Mechanism of action of antioxidants as exemplified in vegetables, tomatoes and tea Weisburger, J.H.
  25. Ann. Clin. Biochem. v.35 Is measuring serum antioxidant capacity clinically useful? Woodford, F.P.;Whitehead, T.P.
  26. Neurobiol. Dis. v.7 Oxidative stress and genetics in the pathogenesis of Parkinson's disease Zhang, Y.;Dawson, V.L.;Dawson, T.M.