The effect of Astragali Radix Ethanol extract on Murine CD4 T cells′ Cytokine Profiles in vitro

  • Hee Kang (Department of Pathology, College of Oriental Medicine, Kyungheee University) ;
  • Bae Hyun Su (Department of Physiology, College of Oriental Medicine, Kyungheee University) ;
  • Ahn Kyoo Seok (Department of Pathology, College of Oriental Medicine, Kyungheee University)
  • Published : 2003.10.01

Abstract

Astragali Radix(AR), one of the strong tonic herbs, is known to improve immunological responses in mice and human. In this study, AR's ai-reinforcing effect was examined in the context of CD4/sup +/ T cells' TCR/CD3 induced activation responses. In order to evaluate the direct effect of AR on helper T cells, CD4/sup +/ T cells are isolated using magnetic bead and their proliferation and CD69 expression in AR treated medium were assessed with anti-CD3/anti-CD28 activation for 48h. CD4 T cells' proliferation was slightly increased but there was little effect on CD69 expression. RT PCR and ELISA equally demonstrated that IL-2 and IL-4 production was increased but IFN-ν was down-regulated. This shows AR ethanol extract favors Th2 cytokine profile under neutral conditions.

Keywords

References

  1. Immunol Rev v.170 Stimulation of naive and memory T cells by cytokines Tough, D.F.;Sun, S.;Zhang, X.;Sprent, J.
  2. Annu Rev Immunol v.14 T cell antigen receptor signal transduction pathways Cantrell, D.
  3. Immunol Today v.18 Cellular environments and apoptosis: tissue microenvironments control activated T-cell death Akbar, A.N.;Salmon, M.
  4. J Immunol v.163 On the lifespan of virgin T lymphocytes Di Rosa F.;Ramaswamy, S.;Ridge, J.P.;Matzinger, P.
  5. Textbook of Herbology Kang Byung-Soo(et al.)
  6. J Ethnopharmocology v.73 Effects of Astragali root and Hedysari root on the murine B and T cell differentiation Qing-Hua Song;Takao Kobayashi;Li-Mei;Tie Hong;Cyong, Jong-Chol
  7. Int. J. Immunopharmac v.19 Immunomodulating activity of Chinese medicinal herbs and Oldenlandia Diffusa in particular Yoshida, Y.;Wang, M.Q.;Liu, J.N.;Shan, N.E.;Yamashita, U.
  8. Biol.Pharm.Bull v.20 no.11 Polysaccharide of Astragali Radix Enhances IgM Antibody Production in Aged Mice Keiji Kajimura;Yasuhiro Takagi;Keiichi Miyano;Yoshiyuki Sawabe;Mayumi Mimura;Yoshikazu Sakagami;Hiroshi Yokoyama;Kaisuke Yoneda
  9. Int. J. Immunopharmac v.21 Stimulating activity of Chinese medicinal herbs on human lymphocyte in vitro Shan, B.E.;Yoshida, Y.;Sugiura, T.;Yamashita, U.
  10. J. Kor. Oriental Oncol-ogy. v.3 Hemopoi-etic and Radioprotective Effects of Several Herbs on Murine Splenocytes and Bone Marrow Cells Lee, Neung-Gi;Choi, Seung-Hoon;Ahn, Kyoo-Seok
  11. Stem Cells v.12 The activation antigen CD69 Ziegler, S.F.;Ramsdell, F.;Alderson, M.R.
  12. Annu Rev Immunol v.15 Differential signaling by lymphocyte antigen receptors Albeorola-Ila;Takaki, S.;Kerner, J.D.;Perlmutter, R.M.
  13. Korean Journal of Oriental Physiology and Pathology v.15 no.4 Astragali Radix increases survival and activity of spleen lymphocytes Kang, Hee;Bae, Hyun-Su;Ahn, Kyoo-Seok
  14. J Immunol v.166 Broad programming by IL-2 receptor signalling foextended growth to multiple cytokines and functional maturation of antigen-activated T cells Malek, T.R.;Yu, A.;Scibelli, P.;Lichtenheld, M.G.;Codias, E.K.
  15. Immunol Today v.17 Interleukin 2 and its receptors: recent advances and new immunological functions Theze, J.;Alzari, P.M.;Bertolio, J.
  16. J Immunol v.136 Two types of murine helper T cell clone; Definition according to profiles of lymphokine activities and secreted proteins Mosmann, T.R.;Cherwinski, H.;Bond, M.W.;Giedlin, M.A.;Coffman, R.L.
  17. Clin Immunol Immunopathol v.84 The Th1/Th2 dichotomy, hsp60 autoimmunity and type I diabetes Cohen, I.R.
  18. Annu Rev Immunol v.17 Transcriptional regulation of T lymphocyte development and function Kuo, C.T.;Leiden, J.M.
  19. Cytokine v.11 Qunatification of murine cytokine mRNAs using real time quantitative reverse transcriptase PCR Overberg, L.;Valckx, D.;Waer, M.;Mathieu, C.
  20. Mol Vis v.6 Gene expression in the mouse retina; the effect of damaging light Grimm, C.;Wenzel, A.;Hafezi, F.;Reme, C.E.