Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Studies on the Antitumor Activity of Gamisoam-san via Suppressing Angiogenesis and Growth Factor Expression

혈관신생 및 이식암세포증식 억제를 통한 가미소암산의 항암작용연구

  • Yoon Sung Chan (Department of Professional Graduate School of Oriental Medicine) ;
  • Ahn Seong Hun (Department of Professional Graduate School of Oriental Medicine) ;
  • Mun Yean Ja (Department of Professional Graduate School of Oriental Medicine) ;
  • Kim Jin Kyeong (College of Medicine, Pochon CHA University) ;
  • Choo Young Kug (Division of Biology Science, College of Natural Science, wonkwang University) ;
  • Jung Kyu Yong (Department of Pharmacology, wonkwang School of Medicine) ;
  • Kim Yeong Mok (Department of Orental Pathology, College of Oriental Medicine, wonkwang University) ;
  • Woo Won Hong (Department of Professional Graduate School of Oriental Medicine, College of Oriental Medicine)
  • 윤성찬 (원광대학교 한의학전문대학원) ;
  • 안성훈 (원광대학교 한의학전문대학원) ;
  • 문연자 (원광대학교 한의학전문대학원) ;
  • 김진경 (포천중문의과대학교 의과대학) ;
  • 추영국 (원광대학교 자연과학대학 생물학교실) ;
  • 정규용 (원광대학교 의과대학 약리학교실) ;
  • 김영목 (원광대학교 한의과대학 병리학교실) ;
  • 우원홍 (원광대학교 한의학전문대학원, 한의과대학 병리학교실)
  • Published : 2003.08.01
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Abstract

Gamisoamsan is a prescription originated in Soamsan which is known as an anti-cancer remedy in the traditional Korean Medicine. To enhance the synergic effects of anti-cancer activity of Soamsan, this study reconstituted the original components of Soamsan with a slight modification and produced a novel herbal remedy, namely Gamisoamsan. To investigate the effects of Gamisoamsan on anti-cancer reaction, I studied the effects of Gamisoamsan on angiogenesis via chorioallantoic membrane (CAM) assay, corneal neovascularization assay and the effects on expression of growth factor which are VEGF, TGF-β, bFGF and IMUP-1. Anti-cancer effects of Gamisoamsan was also abserved through hematological parameters, tumor volume and survival rate in mice. Gamisoamsan inhibited embryonic angiogenesis of blood vessels in CAM assay and inhibited neovascularization of ral cornea. Gamisoamsan reduced cell proliferation in HT1080 cells and IC50 was 2.18 ㎎/㎖ Gamisoamsan reduced the expression of VEGF, TGF-β, bFGF and IMUP-1 which was known as vascular growth factor and this effects of Gamisoamsan was predominant than VP-16. The treatment of Gamisoamsan decreased the CT-26 cell inoculated-tumor volume in mice colon adenocarcinoma and increased mice survival which was inoculated CT-26 cells. The results of the present study suggest that Gamisoamsan extracts has a potential anti-tumor activity and may be an useful remedy to prevent and/or treat cancer.

Keywords

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