Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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The Effects of anti-Alzheimer in pCT105-induced Neuroblastoma cell lines by Radix Polygalae and Rhizoma Acori Graminei mixture extract

원지와 석창포 혼합추출액의 pCT105로 유도된 신경세포암 세포주에 대한 항치매 효과

  • Lee Sung Ryull (Department of Oriental Neuropsychiatry Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Kang Hyung Won (Department of Oriental Neuropsychiatry Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Kim Sang Tae (JuAm of Research Institute Bioscience & Biotechnolo) ;
  • Lyu Yeoung Su (Department of Oriental Neuropsychiatry Medicine, College of Oriental Medicine, Wonkwang University)
  • 이성률 (원광대학교 한의과대학 한방신경정신과학교실) ;
  • 강형원 (원광대학교 한의과대학 한방신경정신과학교실) ;
  • 김상태 (지젠바이오/주압생명공학연구소) ;
  • 류영수 (원광대학교 한의과대학 한방신경정신과학교실)
  • Published : 2003.08.01
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Abstract

Numerous lines of evidence indicate that some of the neurotoxicity associated with Alzheimer's disease (AD) is due to proteolytic fragments of the amyloid precursor protein (APP). Most research has focused on the amyloid 6 (M). However, the possible role of other cleaved products of APP is less clear. Lately It has been reported that a recombinant carboxy-terminal 105 amino acid fragment (CT105) of APP induced strong nonselective inward currents in Xenopus oocyte. In a brain with Alzheimer's disease (AD), to investigate the roles of carboxyl-terminal fragment (CT105) of amyloid precursor protein (APP) in apoptosis processes possibly linked to neurodegeneration associated with AD, we examined the effects of the CT of APP with 105 amino acid residues (CT105) on the alteration of apoptosis triggers in neubroblastoma cells. We have investigated whether Radix Polygalae and Rhizoma Acori Graminei mixture extract (RP+RAG) inhibits CT105-induced apoptosis of neuroblastoma cells. We found that RP+RAG inhibits CT105-induced apoptosis in SK-N-SH cells. Treatment of the cells with RP+RAG inhibited CT105-induced DNA fragmentation and Tunel assay of nuclear chromatin and inhibited the caspase-3 expression in SK-N-SH cells. As the result of this study, In RP+RAG group, the apoptosis in the nervous system is inhibited, the repair against the degerneration of neuroblastoma cells by CT105 expression is promoted. These results indicate that RP+RAG possess strong inhibitory effect of apoptosis in the nervous system and repair effect against the degeneration of neuroblastoma cells by CT105 expression

Keywords

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