Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Gene Expression Profile of the Responder vs. the Non-Responder to the Acupuncture Mediated Analgesic Effects

침자극에 대한 반응과 비반응군 흰쥐의 특이 유전자 발현 연구

  • Sur Young Chan (Department of Physiology, College of Oriental Medicine, Kyunghee University) ;
  • Rho Sam Woong (Department of Physiology, College of Oriental Medicine, Kyunghee University) ;
  • Lee Gi Seog (Department of East-West Medicine, Graduate School, Kyunghee University) ;
  • Ko Eun Jung (Department of Physiology, College of Oriental Medicine, Kyunghee University) ;
  • Hong Moo Chang (Department of Physiology, College of Oriental Medicine, Kyunghee University) ;
  • Shin Min Kyu (Department of Physiology, College of Oriental Medicine, Kyunghee University) ;
  • Min Byung il (Department of East-West Medicine, Graduate School, Kyunghee University) ;
  • Bae Hyun Su (Department of Physiology, College of Oriental Medicine, Kyunghee University, Purimed R&D Institute)
  • 서영찬 (경희대학교 한의과대학 생리학교실) ;
  • 노삼웅 (경희대학교 한의과대학 생리학교실) ;
  • 이기석 (경희대학교 대학원 동서의학과) ;
  • 고은정 (경희대학교 한의과대학 생리학교실) ;
  • 홍무창 (경희대학교 한의과대학 생리학교실) ;
  • 신민규 (경희대학교 한의과대학 생리학교실) ;
  • 민병일 (경희대학교 대학원 동서의학과) ;
  • 배현수 (경희대학교 한의과대학 생리학교실, 퓨리메드(주) 기업부설연구소)
  • Published : 2003.06.01
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Abstract

A pain is the symptom which defends against noxious stimulus about a human body, it is known that if the periphery of perceptive nerve were stimulated by a physical or chemical factors, the stimulation is induced by transmission to pain center in the cerebral cortex according to pain conduction tract. The treatment of pain is to decrease a stimulus that causes a pain or block off a nerve transmitting a stimulus or puts on a way to calm down pain center, but It is for adjustment of a pain to be the most representative in acupuncture among various ways to cure a pain in Oriental medicine. However, the analgesic effect of an individual response to acupuncture stimulation shows marked individual variations, so these days genetic a few approach is attempted. On this the author determined that the responding group was appointed those whose tail flick latency (TFL) responding time delayed the minimum of 30 % comparing with basal reaction time. For those whose TFL time had shorter than 30 % was grouped as a non-responding group. And then the hypothalamus of each group was dissected and RNA was further purified. After synthesizing cDNA using oligo dT primer, products were finally applied to the PCR. The results were as follows; The ratio of responding group to non-responding group was 6:4. Ach T (acetylcholinesterase T subunit), BF-I (Brain factor-I), DBH (Dopamine β-hydroxylase) and PNM (Phosphotidylethanolamine N-Methyltransferase) were revealed significantly in the responding group. Cathepsin B and Tau were revealed significantly in the non-responding group. The PCR results show that Ach T, BF-I, DBH and PNM are expressed abundantly in the responding group, where as cathepsin B and tau are abundant in the non-responding group. These results suggest that the analgesic effect on acupuncture stimulation is related to regulation of neurotransmitter as well as neurodegeration of cerebrum.

Keywords

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